Labelling Review row-Online

Primary Antibodies
Human squamous cell carcinoma, floor of the mouth: immunohistochemical staining for
cytokeratin 17 using NCL-CK17. Note cytoplasmic staining of malignant cells. Paraffin section.
Cytokeratin 18
Clone DC-10
1 mL, 0.1 mL lyophilized NCL-CK18 F P (HIER)
Cytokeratin 18 is normally co-expressed with cytokeratin 8 and is found in
most simple ductal and glandular epithelia.
Product Specific Information
NCL-CK18 reacts with the acidic cytokeratin intermediate filament protein (45
kD) identified as cytokeratin 18. Cytokeratin 18 is reported not to be expressed
in stratified squamous epithelium on most squamous cell carcinomas.
Human colonic adenocarcinoma: immunohistochemical staining for cytokeratin 18 using
NCL-CK18. Note cytoplasmic staining of malignant epithelial cells. Paraffin section.
Cytokeratin 19
Clone b170
1 mL, 0.1 mL lyophilized NCL-CK19 F P (Enzyme)
7 mL Bond ready-to-use PA0799 P (Enzyme)
The smallest human cytokeratin filament protein (40 kD) has been identified
as cytokeratin 19 and has been reported to be expressed in a large number
of epithelial cell types, including many ductal and glandular epithelia.
Product Specific Information
NCL-CK19 produces a complex heterogenous staining pattern in nonkeratinizing
squamous epithelia and hair follicles, with strong staining of the
basal layer observed.
Refer to page 26 for the Bond ready-to-use format.
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Human rectal adenocarcinoma: immunohistochemical staining for cytokeratin 19 using
NCL-CK19. Note cytoplasmic staining of malignant epithelial cells. Paraffin section.
Cytokeratin 20
Clone PW31
1 mL, 0.1 mL liquid NCL-L-CK20-561 P (HIER)
7 mL Bond ready-to-use PA0918 P (HIER)
Clone CK205
1 mL lyophilized NCL-CK20-543 P (HIER)
Clone Ks20.8 1 mL lyophilized NCL-CK20 P (HIER/Enzyme) W
1 mL liquid NCL-L-CK20 P (HIER/Enzyme) W
7 mL ready-to-use RTU-CK20 P (HIER/Enzyme)
Clone PW31 was developed to produce superior staining on paraffin sections.
Cytokeratin 20 has been demonstrated to be almost entirely confined to the
gastric and intestinal epithelium, urothelium and Merkel cells of the skin.
Cytokeratin 20 is less acidic than other type I cytokeratins and is of interest
due to its restricted tissue expression. In normal tissue, cytokeratin 20 is
expressed in intestinal epithelium, gastric foveolar epithelium, a number of
endocrine cells in the upper portions of the pyloric glands, urothelium and
Merkel cells in epidermis. In tumors it is reported, there is a marked
difference in the expression of cytokeratin 20 within different carcinomas.
Neoplasms expressing cytokeratin 20 are derived from normal epithelia
which themselves expressed cytokeratin 20. Colorectal carcinomas consistently
express cytokeratin 20, while gastric adenocarcinomas express
cytokeratin 20 to a lesser degree. Adenocarcinomas of the gall bladder and
bile duct, ductal cell adenocarcinomas of the pancreas, mucinous ovarian
tumors, Merkel cell tumors and transitional cell carcinomas have also been
reported to express cytokeratin 20.
Refer to page 26 for the Bond ready-to-use format.
Human colonic adenocarcinoma: immunohistochemical staining for cytokeratin 20 using
NCL-L-CK20-561. Note staining of malignant cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range