Labelling Review row-Online

Primary Antibodies
Human Kaposi's sarcoma: immunohistochemical staining for HHV8 latent nuclear antigen
using NCL-HHV8-LNA. Note nuclear staining in a proportion of infected tumor cells. Paraffin
section.
Human Neutrophil Defensins (1/2/3)
Clone D21
1 mL lyophilized NCL-DEFENSIN P (HIER)
Defensins are antimicrobial agents which together with serprocidins,
lysozyme, bacterecins, protegrins and indolicidin have been isolated from
neutrophil and macrophage granules. Defensins are synthesized as 93 to 96
amino acid pre-propeptides. In fully differentiated phagocytes, virtually all of
the cellular defensin exists as processed mature peptide. Neutrophil
defensins are stored in azurophil granules which discharge their contents
into microbe-containing phagosomes through the process of phagosome/
granule fusion. Paneth cells of the small intestine are also reported to
secrete defensins, as well as lysozyme into the crypt lumen which may limit
local microbial proliferation and colonization. These peptides may also exert
chemotactic and immunomodulating effects in host defence and
inflammation. The three principle human neutrophil defensin peptides, HNP
1, 2 and 3, are reported to be unique to neutrophils and account for 99
percent of the defensin content in these cells. Activation of neutrophils
leads to a rapid release of HNP which may also be measured in plasma and
other body fluids in infection and inflammation.
Human tonsil: immunohistochemical staining for human neutrophil defensins using NCL-
DEFENSIN. Note intense granular cytoplasmic and extracellular staining of neutrophils.
Paraffin section.
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Human Securin
Clone DCS-280.2
1 mL lyophilized NCL-SECURIN P (HIER)
Human securin (hsecurin), also known as pituitary tumor-transforming gene-
1 (PTTG) product, is required for chromosomal stability in human cells.
Abnormalities of chromosome number are reported to be amongst the most
common genetic aberrations in cancer. The mechanisms for regulating
mitotic chromosome transmission in mammalian cells are, therefore, of
great interest. Human cells without an hsecurin gene lose chromosomes at
a high rate. These losses have been linked to abnormal anaphases during
which cells undergo repeated unsuccessful attempts to segregate their
chromosomes. Therefore, human securin is essential for the maintenance of
euploidy. The expression of hsecurin is reported to correlate with cell
proliferation in a cell cycle-dependent manner in both normal tissues and in
several tumor types. hsecurin specifically binds to Ku, the regulatory subunit
of the DNA-dependent protein kinase. Ku and hsecurin associate both in
vitro and in vivo. DNA double-strand breaks prevent Ku/hsecurin association
showing that genome damaging events can result in the induction of
pathways that activate DNA repair mechanisms and halt cell cycle
progression. It has also been proposed that hsecurin connects DNAdamage
response pathways with sister chromatid separation delaying
mitosis while DNA repair occurs.
Human tonsil: immunohistochemical staining for human securin using NCL-SECURIN.
Note nuclear staining of proliferating cells. Paraffin section, nickel DAB.
Human Spasmolytic Polypeptide
Clone GE16C
1 mL lyophilized NCL-HSP P (HIER)
Human spasmolytic polypeptide (HSP) is a member of the trefoil peptide
family which is reported to be expressed in discrete regions of the body,
most notably the gastrointestinal tract. In the stomach, HSP is reported to be
localized to foveolar and surface epithelium, pyloric glands and mucous
neck cells.
Normal human stomach: immunohistochemical staining of human spasmolytic polypeptide
using NCL-HSP. Note cytoplasmic staining of cardiac glands. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.