Labelling Review row-Online

ISH Reagents/Probes
Control Probe (Fluorescein-Conjugated)
50 Tests, 10 Tests liquid probe NCL-CONTROL P
Product Specific Information
NCL-CONTROL has been produced by labeling randomly generated
oligonucleotide sequences with fluorescein using the same procedures as
applied to the mRNA specific oligonucleotide probes from Leica
Microsystems. Therefore, NCL-CONTROL is ideally suited for use as a
negative control alongside RNA specific probes providing confirmation of the
staining pattern obtained by these specific oligonucleotide probes.
Cytomegalovirus Probe
(Fluorescein-Conjugated)
50 Tests liquid probe NCL-CMV P
Cytomegalovirus (CMV) infection may occur in lung, kidney, gut and other
organs of individuals who are immunologically immature, such as the fetus
and neonate. CMV infection also occurs in situations of immunosuppression
such as transplant recipients, individuals undergoing chemotherapy and
those with HIV infection.
Product Specific Information
NCL-CMV detects an early gene RNA transcript which is expressed in
permissive infection.
Epstein-Barr virus Probe
(Fluorescein-Conjugated)
50 Tests,10 Tests liquid probe NCL-EBV P
EBV infection is associated with a variety of pathological conditions. The
virus has been reported to be demonstrated in infectious mononucleosis,
Burkitt's lymphoma, the Reed Sternberg cells of Hodgkin's disease and in
nasopharyngeal carcinoma. In HIV infection, EBV has also been reported to
be demonstrated in primary CNS lymphomas and oral hairy leukoplakia
lesions.
Product Specific Information
NCL-EBV is a fluorescein-labelled oligonucleotide cocktail of probes
designed to demonstrate cells latently-infected with EBV. The probe
hybridizes to abundantly expressed Epstein-Barr virus-encoded RNA (EBER)
transcripts which are concentrated in the nuclei of latently-infected cells.
These transcripts are thought to block the activation of dsRNA-dependent
eukaryotic initiation factor 2a (elF-2a) protein kinase DAI. In the absence of
EBER, elF-2a inhibits cellular protein synthesis.
Human nasopharyngeal carcinoma: in situ hybridization for Epstein-Barr virus (EBV) encoded
RNA (EBER) using NCL-EBV. Note intense staining of EBV-infected cells. Paraffin section.
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Epstein-Barr virus Probe ISH Kit
50 Tests kit NCL-EBV-K P
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Epstein-Barr virus encoded RNA (EBER) is reported to be present in both
latent and lytic EBV infection. These transcripts are thought to block the
activation of dsRNA-dependent eukaryotic initiation factor 2a (elF-2a)
protein kinase DAI.
Product Specific Information
NCL-EBV-K contains a fluorescein-labelled oligonucleotide cocktail for the
detection of mRNA sequences contained in 1 mL of hybridization solution
sufficient to stain 50 preparations. A control probe is a fluorescein-labelled
random oligonucleotide cocktail contained in 1 mL of hybridization solution
which is also included. The control probe is ideally suited for use as a
negative control alongside the EBV probe. Other reagents include 500 �g of
lyophilized Proteinase K, anti-fluorescein isothiocyanate conjugated to
alkaline phosphatase, 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro
blue tetrazolium (NBT) in dimethylformamide solution, levamisole
hydrochloride and 3-aminopropyltriethoxysilane (APES)-coated slides.
Histone Probe (Fluorescein-Conjugated)
50 Tests liquid probe NCL-HISTONE-513 P
The synthesis of histone mRNA is closely coupled with DNA replication.
During the S phase of the cell cycle the level of histone mRNA increases
over fifty fold then rapidly disappears at the start of the G2 phase. These
changes are produced by rapid mRNA degradation as well as modulation of
transcription. The presence, therefore, of abundant quantities of histone
mRNA provides an indicator of cycling cells which can be easily detected.
Product Specific Information
NCL-HISTONE-513 is a fluorescein-labelled oligonucleotide cocktail for the
detection of histone, H2b, H3 and H4 mRNA sequences contained in 1 mL of
hybridization solution sufficient for in situ hybridization.
Human breast carcinoma: in situ hybridization for histone mRNA using NCL-HISTONE-513.
Note intense staining of proliferating tumor cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
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