Labelling Review row-Online

Buffers
Epitope Retrieval Solution pH9
1 L pH9 (x10 Concentrate) RE7119 P (HIER)
Novocastra Epitope Retrieval Solutions are intended for Heat Induced
Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue
sections as part of an immunohistochemical procedure. HIER using an
appropriate pH solution improves the staining of some antibodies by
exposing epitopes within tissue that has been masked during fixation. The
development of Epitope Retrieval using heat was first reported in 1991 by Shi
S-R et al. Journal of Histochemistry and Cytochemistry 39: 741-748 (1991).
Since then numerous studies have been published looking at the effects of
molarity, pH and heating methods on epitope retrieval. A universal HIER
technique suitable for all epitopes does not exist. A combination of different
heating methods and epitope retrieval solutions may be used to optimize
unmasking of antigens where this technique is recommended. HIER is not
recommended for all antibodies. Optimum conditions for epitope retrieval
should be validated by the user, as these are dependant upon tissue, fixation
and/or primary antibody. RE7119 and RE7224 are supplied as a 1 L volume,
sufficient to prepare 10 L of working solution.
Kidney pre-treated with Epitope Retrieval Solution pH9 (RE7119). Staining for Wilms' tumor
protein using NCL-L-WT1-562. Paraffin section.
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Enzyme Proteinase K (IHC)
100 mL kit RE7160-K P (Enzyme)
IVD IVD
Enzyme pretreatment of formalin-fixed, paraffin-embedded tissue sections
improves the staining of some antibodies by exposing epitopes within tissue
that have been masked during fixation. The first proteolytic enzyme
employed for epitope retrieval was trypsin. More recently, proteinase K
which is commonly used in in situ hybridization techniques has been
reported to be of use.
Product Specific Information
Novocastra Enzyme Proteinase K (IHC), RE7160-K, is intended for the
enzymatic pretreatment of formalin-fixed, paraffin-embedded tissue sections
prior to incubation with a primary antibody in an immunohistochemical (IHC)
procedure. This product can be used for epitope retrieval with Novocastra
antibodies for which trypsin is recommended, known exceptions to this are
NCL-C-JEJUNI, NCL-BrdU, NCL-CYCLIN D1, NCL-COLL-IIp, and NCL-CYCLIN
D1-GM. This two part kit comprises 0.75 mL of Enzyme Proteinase K
Concentrate, RE7126, and 100 mL of Enzyme Proteinase K Buffer, RE7127,
sufficient to produce 100 mL of working strength enzyme solution. This
product is used in an IHC procedure, which allows the qualitative
identification by light microscopy. Epitope retrieval by enzymatic
pretreatment is recommended for a limited number of antibodies. Optimum
conditions for epitope retrieval should be validated by the user as these are
dependent upon tissue, fixation and/or primary antibody.
Liver pre-treated with Enzyme Proteinase K (RE7160-K). Staining for Cytokeratin 8/18 using
NCL-L-5D3. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.