Labelling Review row-Online

Primary Antibodies
Immunoglobulin D
Clone DRN1C
1 mL, 0.1 mL liquid NCL-L-IgD P (HIER) Reference Range
Clone DRN1C was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
IgD, together with IgM, are the major immunoglobulins expressed on the
surface of B cells where it seems they may operate as mutually interacting
antigen receptors for the control of lymphocyte activation and suppression.
The greater susceptibility of IgD to proteolysis in combination with antigen
could well be implicated in such a function.
Product Specific Information
The use of PBS-based diluents may result in increased background staining.
Clone DRN1C was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
Human tonsil: immunohistochemical staining for Immunoglobulin D using NCL-L-IgD. Note
intense membrane staining of B cells. Paraffin section.
Immunoglobulin G
Clone RWP49 New!
1 mL, 0.1 mL liquid NCL-L-IgG P (HIER)
Clone RWP49 was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
The human immunoglobulins consist of two identical heavy chains (~50 kD) and
two identical light chains, which are linked together by disulphide bonds. The
light chains can be either kappa or lambda. The five immunoglobulins IgA, IgD,
IgE, IgG and IgM differ in their heavy chains, and IgA and IgM differ as they can
occur in polymeric forms. The heavy chain of IgG is named the gamma-chain. In
humans, IgG consists of four sub classes that differ only marginally in their
amino acid composition. Antibodies to IgG have been reported to be useful in
the identification of plasma cells, lymphoid cells containing IgG and classifying
B cell derived neoplasms. The normal B cell population is polyclonal,
expressing a range of different immunoglobulins. In contrast, the majority of B
cell neoplasms are characterized by the proliferation of monoclonal cells
expressing one type of light chain, whereas more than one type of heavy chain
can be expressed by the same cell. IgG positive neoplasms include hairy cell
leukemia, splenic lymphoma and follicular lymphoma.
Human tonsil: immunohistochemical staining for Immunoglobulin G using NCL-L-IgG. Paraffin
section.
/ 124
For detailed information on all products please visit our website:
www.leica-microsystems.com
Immunoglobulin M
Clone 8H6
1 mL, 0.1 mL liquid immunoglobulin M NCL-L-IgM P (HIER) W
Reference Range
Clone 8H6 was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
IgM, together with IgD, is the major immunoglobulin expressed on the
surface of B cells and normally constitutes about 10 per cent of serum
immunoglobulin. IgM antibody is prominent in early immune responses to
most antigens and predominates in certain antibody responses such as
‘natural’ blood group antibodies.
Human tonsil: immunohistochemical staining for immunoglobulin M using NCL-L-IgM.
Note staining of follicular dendritic cells, mantle zone B cells and intense staining of plasma
cells. Paraffin section.
Immunoglobulin Antibodies
Polyclonal
1 mL lyophilized immunoglobulin A
NCL-IgAp P (Enzyme) W
Polyclonal
1 mL lyophilized immunoglobulin D
NCL-IgDp P (Enzyme)
Polyclonal
1 mL lyophilized immunoglobulin G
NCL-IgGp P (Enzyme) W
Polyclonal
1 mL lyophilized immunoglobulin M
NCL-IgMp P (Enzyme) W
The basic structure of immunoglobulin (Ig) molecules is a tetramer of two
light chains and two heavy chains linked by disulfide bonds. There are two
types of light chains, kappa and lambda, each composed of a constant
domain (CL) and a variable domain (VL). There are five types of heavy
chains: alpha, delta, epsilon, gamma and mu, all consisting of a variable
domain (VH) and three (in alpha, delta and gamma) or four (in epsilon and
mu) constant domains (CH1 to CH4).
Product Specific Information
NCL-IgAp, NCL-IgDp, NCL-IgGp and NCL-IgMp have each been solid-phase
absorbed to remove cross-reactivity.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.