Labelling Review row-Online

Human von Willebrand Factor
(Factor VIII-related antigen)
Clone 36B11
1 mL, 0.1 mL lyophilized NCL-vWF F P (HIER)
1 mL liquid NCL-L-vWF F P (HIER)
7 mL Bond ready-to-use PA0400 P (HIER)
Human von Willebrand factor (or factor VIII-related antigen) is a 270 kD
multimeric plasma glycoprotein. It mediates platelet adhesion to injured
vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
The von Willebrand factor has functional binding domains to platelet
glycoprotein Ib, glycoprotein Ib/IIIa, collagen and heparin. von Willebrand
factor is synthesized by endothelial cells and is reported to be expressed in
a number of tumors of vascular origin.
Refer to page 43 for the Bond ready-to-use format.
Hypoxia Inducible Gene 2 Protein
Clone HX34Y New!
1 mL, 0.1 mL liquid NCL-L-HIG2 P (HIER)
The gene encoding hypoxia-inducible gene 2 protein (HIG2) is one of the
transcriptional targets for the activated beta-catenin/Tcf-4 complex and its
product functions as an autocrine growth factor that enhances cell growth.
This gene encodes a trans-membrane protein of 7 kD molecular weight that
was found to be expressed exclusively in renal cell carcinomas (RCC) and
fetal kidney. Reports indicate that ELISA analysis of clinical samples
identified secretion of HIG2 protein into plasma of RCC patients even at an
early stage of tumor development. HIG2 expression is reported to be
expressed at higher levels in ovarian clear cell carcinomas when compared
to those of clear cell renal tumors.
Human clear cell renal cell carcinoma: immunohistochemical staining for hypoxia inducible
gene 2 protein using NCL-L-HIG2.
ICAM-1 (CD54)
Clone 23G12
1 mL, 0.1 mL lyophilized NCL-CD54-307 P (HIER)
The CD54 (ICAM-1) is an integral membrane glycoprotein of 85 to 110 kD with
seven potential N-linked glycosylation sites. The antigen is reported to be
expressed on monocytes and endothelial cells. Expression of the CD54
glycoprotein can be induced or upregulated on many cell types including
B and T lymphocytes, thymocytes, fibroblasts, keratinocytes and epithelial
cells. The CD54 antigen is important in mediating immune and inflammatory
responses. It mediates the adhesion of T cells with antigen-presenting cells
and is involved in T cell to T cell and T cell to B cell interactions.
Human peripheral lung: immunohistochemical staining for CD54 antigen (ICAM-1) using
NCL-CD54-307. Note membrane staining of epithelial cell surfaces of the air spaces.
Paraffin section.
Immunoglobulin A
Clone N1CLA
1 mL, 0.1 mL liquid NCL-L-IgA P (HIER) W Reference Range
Clone N1CLA was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
IgA is a member of the antibody class of the immunoglobulin superfamily.
There are several classes and subclasses (isotypes) of antibody, the
antibody isotype being defined by the immunoglobulin heavy chain present
in the molecule. The basic structure of an immunoglobulin molecule consists
of two identical heavy chains (�������������) and two identical light chains,
either kappa or lambda. IgA contains the �-chain and may be present in a
serum or secretory form. In serum, 90 percent of IgA is monomeric, while in
its secretory form it is the main immunoglobulin found in secretions
including tears, saliva, intestinal and bronchial mucous, sweat, colostrum,
and secretions from the prostate and respiratory epithelia, where it has the
job of defending exposed external surfaces of the body against attack from
micro organisms. Secretory IgA is synthesized locally by plasma cells and
dimerized intracellularly with a cysteine-rich J-chain.
Product Specific Information
Clone N1CLA was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
Human appendix: immunohistochemical staining for immunoglobulin A using NCL-L-IgA. Note
intense staining of plasma cells and secreted immunoglobulin A. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies