Labelling Review row-Online

Primary Antibodies
Giardia intestinalis
Clone 9D5.3.1
1 mL, 0.1 mL lyophilized NCL-GI P (HIER)
Giardia intestinalis (formerly Giardia lamblia) is a flagellated protozoan,
which infects humans via contaminated water supplies, causing illnesses
ranging from acute severe bloody diarrhoea, through moderate enteritis,
chronic diarrhoea with malabsorption, to asymptomatic excretion. The
remarkable hardiness of the cyst form and the low numbers required to
infect make the epidemiology uncertain, although water-based infections
are the most common.
Glial Fibrillary Acidic Protein
Clone GA5
1 mL, 0.1 mL lyophilized NCL-GFAP-GA5 FP
7 mL Bond ready-to-use PA0026 P (HIER)
Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of
52 kD reported to be expressed in glial cells eg astrocytes and ependymal
cells. In the peripheral nervous system, GFAP has been reported to be
expressed in Schwann cells, enteric glial cells and satellite cells of human
sensory ganglia and in neoplastic tissues GFAP has been reported to be
expressed in astrocytomas and ependymomas.
Product Specific Information
When using NCL-GFAP-GA5 the heat induced epitope retrieval (HIER)
technique may improve staining in some cases.
Refer to page 30 for the Bond ready-to-use format.
Glucagon
Polyclonal
0.5 mL lyophilized NCL-GLUCp FP
Polyclonal
7 mL Bond ready-to-use PA0594 P (HIER)
Glucagon expression has been reported in the endocrine cells of the
pancreatic islets and also in the mucosa of small and large intestine.
Pancreatic glucagon, a peptide of 29 amino acids, has biological activities
including glycogenolysis, lipolysis, gluconeogenesis and ketogenesis. These
effects are all antagonistic to insulin action and, therefore, lead to increased
blood sugar levels. The majority of glucagonomas are reported to arise from
the pancreas and produce pancreatic glucagon. These tumors are found
chiefly in the main body or tail of the pancreas.
Refer to page 30 for the Bond ready-to-use format.
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Human pancreas: immunohistochemical staining for Glucagon using NCL-GLUCp.
Note cytoplasmic staining in the endocrine cells of the islets. Paraffin section.
Glucocorticoid Receptor
Clone 4H2
1 mL lyophilized NCL-GCR P (HIER) W
The glucocorticoid receptor of molecular weight 90 kD has three main
functional regions; the N-terminal modulating region, the DNA binding
region and the C-terminal steroid binding region. The glucocorticoid
receptor is reported to be widely distributed and expressed in many cultured
cell lines eg CEM-C7. Glucocorticoid receptor is reported to be expressed in
neoplastic cells of chronic lymphocytic leukemia (CLL). Two isoforms of
glucocorticoid receptor exist; alpha and beta, with the alpha form usually
the most abundant. The control of gene expression by glucocorticoids has
been widely studied as a model for transcriptional regulation. Glucocorticoid
receptors are reported to induce or repress the expression of genes in
response to glucocorticoids, mediating such processes as cell growth,
differentiation and apoptosis. Glucocorticoid receptors may also form a
complex with heat shock protein 90 and in certain instances render the nonligand
bound receptor transcriptionally inactive.
Product Specific Information
NCL-GCR is raised to the N-terminal modulating region.
Human tonsil: immunohistochemical staining for glucocorticoid receptor using NCL-GCR.
Note nuclear staining in a wide distribution of cell types. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.