Labelling Review row-Online

Human acute lymphoblastic leukemia: immunohistochemical staining for Pax-5 using
NCL-L-PAX-5. Note nuclear staining of B cells. Paraffin section.
P-Cadherin
Clone 56C1
1 mL lyophilized NCL-P-Cad F P (HIER) W
1 mL liquid NCL-L-P-Cad F P (HIER) W
P-cadherin, like E-cadherin, is a Ca 2+ -dependent cell adhesion molecule
and has a fundamental role in maintaining the integrity of multicellular
structures. It is responsible for selective cell to cell adhesion. P-cadherin
expression is reported to be restricted and the protein is only detected in the
basal or parabasal layers of stratified epithelia. P-cadherin may contribute
to the maintenance of the epithelial phenotype and be involved in the final
stage of tumor progression in epidermal carcinomas. Changes in the pattern
of P-cadherin expression have also been reported in breast and melanocytic
cancers.
Human placenta: immunohistochemical staining for P-cadherin using NCL-L-P-Cad.
Note intense membrane staining of cytotrophoblasts. Paraffin section.
PECAM-1 (CD31)
Clone 1A10
1 mL, 0.1 mL lyophilized NCL-CD31-1A10 P (HIER)
7 mL Bond ready-to-use PA0250 P (HIER)
See also CD31 (PECAM-1) on page 77.
Perforin
Clone 5B10
1 mL, 0.1 mL lyophilized NCL-PERFORIN P (HIER)
Perforin is a pore-forming protein found in cytoplasmic granules of cytotoxic
T-lymphocytes (CTLs). CTLs bind to cells which express foreign antigens and
induce them to lyse. Perforin forms circular lesions on the target cell
membrane similar to those induced by complement. Perforin and C9 share a
high degree of homology particularly at the membrane spanning region.
Perforin is reported to be constitutively expressed in human CD3 negative,
CD56 positive NK cells, CD3 positive large granular lymphocytes and
gamma/delta T cells. This expression is significantly induced in CD8 positive
T cells but to a lesser extent in gamma/delta T cells and NK cells. The
induction of perforin mRNA is partially blocked by the immunosuppressive
drug cyclosporin A.
Human follicular lymphoma: immunohistochemical staining for perforin using NCL-PERFORIN.
Note focal granular staining of occasional cytotoxic T lymphocytes. Paraffin section.
Peripherin
Clone PJM50
1 mL lyophilized NCL-PERIPH F P (HIER) W
Peripherin is a 57 kD type III intermediate filament protein that is expressed
in peripheral neurons, including enteric ganglion cells. Peripherin is
expressed in the developing peripheral nervous system and is highly
enriched in neuronal derivatives of the neural crest. The expression or
absence of peripherin may be used to demonstrate abnormalities of the
enteric nervous system. The assessment of the density of ganglion cells is of
importance in Hirschsprung's disease (HD)-related disorders. Peripherin is
also reported to be expressed in neural crest derived tumors such as
neuroblastomas and ganglioneuroblastomas.
Human small bowel: immunohistochemical staining for peripherin using NCL-PERIPH.
Note intense cytoplasmic staining of enteric ganglion cells and neural elements.
Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies