Labelling Review row-Online

Bond Ready-to-Use Primary Antibodies
B Cell Specific Octamer Binding
Protein-1 (BOB-1)
Clone TG14
7 mL Bond ready-to-use PA0558 P (HIER)
For In Vitro Diagnostic Use
B cell specific octamer binding protein-1 (BOB-1), also known as OBF-1 and
OCA-B, is a lymphocyte specific transcriptional coactivator protein. It
interacts with OCT1 and OCT2 transcription factors and contributes to the
transcriptional activity of octamer motifs. BOB-1 has been reported to be
detectable in all B cell populations found in reactive lymphoid tissues. The
strongest expression is found in germinal center B cells and plasma cells.
The expression of BOB-1 in B cell tumors has been reported to be variable.
Also available as a Novocastra concentrate, refer to page 59.
Lymphoma: immunohistochemical staining with Bond ready-to-use BOB-1 (TG14) using Bond
Polymer Refine Detection.
Bcl-2 Oncoprotein
bcl-2/100/D5 New!
7 mL Bond ready-to-use PA0117 P (HIER)
For In Vitro Diagnostic Use
Bcl-2 is a member of a family of proteins that are involved in apoptosis. Bcl-2
is an integral inner mitochondrial membrane protein of 25 kD and has a wide
tissue distribution. It is considered to act as an inhibitor of apoptosis. For this
reason, Bcl-2 expression is inhibited in germinal centers where apoptosis
forms part of the B cell production pathway. In 90 percent of follicular
lymphomas a translocation occurs which juxtaposes the Bcl-2 gene at
18q21, to an immunoglobulin gene. This t(14;18) translocation can deregulate
gene expression and Bcl-2 over-expression can be demonstrated immunohistochemically
in the vast majority of follicular lymphomas.
Also available as a Novocastra concentrate, refer to page 60.
Tonsil: immunohistochemical staining with Bond ready-to-use Bcl-2 (bcl-2/100/D5) using Bond
Polymer Refine Detection.
Bcl-6
Clone LN22
7 mL Bond ready-to-use PA0204 P (HIER)
For In Vitro Diagnostic Use
Bcl-6 is an oncoprotein that plays a role in the differentiation of normal
germinal center B cells. The Bcl-6 antigen is an important marker of
follicular, diffuse large B cell and Burkitt's lymphoma and also nodular,
lymphocyte predominant Hodgkin's disease.
Product Specific Information
Bcl-6 (LN22) is recommended as part of a panel of antibodies in the
characterization of lymphomas of B cell origin.
Also available as a Novocastra concentrate, refer to page 60.
Tonsil: immunohistochemical staining with Bond ready-to-use Bcl-6 (LN22) using Bond
Polymer Refine Detection.
Beta-Catenin
Clone 17C2 New!
7 mL Bond ready-to-use PA0083 P (HIER)
For In Vitro Diagnostic Use
The catenins, (alpha, beta and gamma) are cytoplasmic proteins which bind
to the highly conserved tail of the E-cadherin molecule. Beta-catenin is a
component of the adherens junction, a multiprotein complex which supports
Ca2+ -dependent cell to cell contact which in itself is critical for adhesion,
signal transmission and for anchoring the actin cytoskeleton. Beta-catenin’s
role is as a transcription effector of the wnt-signalling pathway.
Immunohistochemistry is the best way to demonstrate nuclear expression of
beta-catenin and wnt-pathway activation. This aberrant expression is
observed in human tumorigenesis and especially in colorectal cancer.
Also available as a Novocastra concentrate, refer to page 62.
Colon polyp: immunohistochemical staining with Bond ready-to-use Beta-Catenin (17C2) using
Bond Polymer Refine Detection.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Bond