Labelling Review row-Online
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Bond<br />
Detection Systems<br />
Bond Polymer Refine Detection<br />
300 Tests (100 μL dispense) or<br />
200 Tests (150 μL dispense) DS9800 P<br />
For In Vitro Diagnostic Use<br />
Components<br />
A state-of-the-art Compact Polymer detection system for use in both<br />
immunohistochemistry and chromogenic in situ hybridization. Small<br />
multifunctional linkers enhance tissue penetration, producing unsurpassed<br />
sensitivity. The system is biotin-free.<br />
Bond Polymer Refine Detection contains a peroxide block, post primary,<br />
polymer reagent, DAB chromogen and hematoxylin counterstain. It is<br />
supplied ready-to-use for the automated Bond system.<br />
Application<br />
Immunohistochemistry (IHC)<br />
Primary antibody binding to tissue sections can be visualized using Bond<br />
Polymer Refine Detection, where it provides intense, high resolution<br />
staining. A range of Bond ready-to-use primary antibodies are available, or<br />
alternatively, use antibody concentrates diluted with Bond Primary Antibody<br />
Diluent (AR9352).<br />
Colon mucosa: immunohistochemical staining with Bond ready-to-use Cytokeratin 8/18 (5D3)<br />
(PA0067) using Bond Polymer Refine Detection.<br />
Application<br />
Chromogenic in Situ Hybridization (ISH)<br />
Bond Polymer Refine Detection produces highly specific, sensitive and<br />
reproducible demonstration of nucleic acid sequences through controlled<br />
hybridization reactions.<br />
Bond ready-to-use ISH probes (fluorescein/biotin labeled along with Anti-<br />
Fluorescein/biotin secondary antibodies) are used in conjunction with Bond<br />
Polymer Refine Detection to produce perfectly tuned, optimized ISH staining<br />
results on the Bond system.<br />
Hodgkin's lymphoma: in situ hybridization staining for Epstein-Barr virus (EBV) encoded mRNA<br />
with EBER Probe (PB0589) using Bond Polymer Refine Detection.<br />
/12<br />
For detailed information on all products please visit our website:<br />
www.leica-microsystems.com<br />
Bond Polymer Refine Red Detection<br />
100 Tests DS9390 P<br />
For In Vitro Diagnostic Use<br />
Bond Polymer Refine Red Detection is an IVD labeled red detection system for<br />
the automated Bond system. Bond Polymer Refine Red Detection is biotinfree,<br />
utilizing alkaline phosphatase (AP)-linked compact polymers to provide<br />
enhanced tissue penetration and unsurpassed reagent sensitivity. It contains<br />
post primary, polymer reagent, Fast Red chromogen, and hematoxylin<br />
counterstain and is supplied in a convenient, ready-to-use format.<br />
Human skin stained for melanoma marker HMB45 with NCL-HMB45 using Bond Polymer Refine<br />
Red Detection. Note intense cytoplasmic staining of melanocytes in contrast to the b<strong>row</strong>n<br />
endogenous melanin.<br />
Bond Intense R Detection<br />
300 Tests (100 μL dispense) or<br />
200 Tests (150 μL dispense) DS9263 PF<br />
For Research Use Only<br />
Components<br />
Bond Intense R Detection is a peroxidase detection system optimized for<br />
use on the automated Bond system and is ideal for research applications. It<br />
contains a peroxide block, streptavidin/peroxidase conjugate, DAB<br />
chromogen and hematoxylin counterstain. Users must supply a biotinylated<br />
secondary antibody of their choice.<br />
Application<br />
By allowing a free choice of biotinylated secondary antibody, Bond Intense<br />
R Detection is ideal for the detection of primary antibodies from any species.<br />
Research applications such as IHC staining of mouse tissues can be<br />
accommodated in this manner. The intense deposition of DAB reaction product<br />
produces strong immunostaining.<br />
Bond Research Detection<br />
300 Tests (100 μL dispense) or<br />
200 Tests (150 μL dispense) DS9455<br />
For Research Use Only<br />
Components<br />
The Bond research functionality is created together with a research dongle<br />
and an "open" Bond Research Detection from Leica Microsystems. This<br />
open detection system consists of six standard 30 mL open containers in a<br />
reagent tray. The tray is registered on Bond like any other detection system<br />
(one barcode only), but each of the containers can be configured with<br />
reagent of the user's choice.<br />
Application<br />
Bond Research Detection offers researchers the ability to tailor applications and<br />
fully automate for ease of use.<br />
Products in this catalog are subject to regulatory approval.<br />
This catalog is not for use in the USA.