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Primary Antibodies
CD44 Variant Antibodies
Clone VFF-327v3
1 mL lyophilized CD44 variant 3 NCL-CD44v3 F P (HIER) W
Clone VFF-8
1 mL lyophilized CD44 variant 5 NCL-CD44v5 F P (HIER)
Clone VFF-7
1 mL lyophilized NCL-CD44v6 F P (HIER)
The CD44 molecule belongs to a family of cellular adhesion molecules found
on a wide range of normal and malignant cells in epithelial, mesothelial and
hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are
normally expressed to encode the basic CD44 (H-CAM) molecule. The
additional 10 exons (v1 to v10) are only expressed by alternative splicing of
the nuclear RNA. The expression of specific cell adhesion molecule CD44
splice variants has been reported to be associated with metastasis in
certain human malignancies, such as breast cancer. A complex pattern of
CD44 variant expression in different tumors compared to the CD44
expression of the normal cell of origin has been reported (Fox SB et al.,
Cancer Research (53): 4539-4546, (1994)). High levels of expression were
observed with the variant exons by breast carcinomas that arise from breast
ductal epithelium which do not normally express CD44. Conversely, normal
gastrointestinal epithelium were reported to express low levels of many of
the CD44 variants and the derived colon cancers expressed low and
variable levels of the variants. Respiratory epithelium which expressed
variants at high levels in normal cells were reported to express the same
variants at similar levels in lung carcinomas.
Human squamous cell carcinoma, floor of the mouth: immunohistochemical staining for CD44
variant 3 using NCL-CD44v3. Note intense membrane staining of tumor cells. Paraffin section.
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CD45
Clone X16/99
1 mL, 0.1 mL lyophilized NCL-LCA F P (HIER) C
7 mL Bond ready-to-use PA0042 P (HIER)
RP2/18, Clone RP2/22
1 mL lyophilized NCL-LCA-RP FP
1 mL liquid NCL-L-LCA-RP FP
7 mL ready-to-use RTU-LCA-RP FP
The CD45 antigen (leukocyte common antigen) is a family of five or more
high molecular weight glycoproteins present on the surface of the majority
of the human leukocytes (including lymphocytes, monocytes and
eosinophils) but absent from erythrocytes and platelets. Various isoforms of
CD45 are generated by alternative splicing of three exons. Expression of
CD45 is necessary for signalling through the T cell receptor. NCL-LCA-RP is
a cocktail of two antibodies, clone RP2/18 and RP2/22.
Product Specific Information
The heat induced epitope retrieval (HIER) technique may enhance staining in
some cases with NCL-LCA-RP, NCL-L-LCA-RP and RTU-LCA-RP.
Refer to page 22 for the Bond ready-to-use format.
Human stomach, B cell lymphoma: immunohistochemical staining for CD45 antigen using
NCL-L-LCA-RP. Note intense membrane staining of tumor cells. Paraffin section.
CD45RA
Clone X148
1 mL lyophilized NCL-B1 FPC
The CD45R subfamily comprises a restricted form of the leukocyte common
antigen and is divided into four isoforms: CD45RA, CD45RB, CD45RC and
CD45RO. The CD45RA molecule, a 220 kD isoform of CD45, is reported to be
expressed on B cells, monocytes and a small proportion of T cells.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range