Labelling Review row-Online

Normal human tonsil: immunohistochemical staining for CD3 antigen using NCL-L-CD3-565.
Note intense membrane staining of T lymphocytes. Paraffin section.
CD4
Clone 4B12
1 mL, 0.1 mL lyophilized NCL-CD4-368 F P (HIER) W
1 mL liquid NCL-L-CD4-368 F P (HIER)
7 mL Bond ready-to-use PA0368 P (HIER)
Clone 1F6
1 mL, 0.1 mL lyophilized NCL-CD4-1F6 P (HIER) W
1 mL liquid NCL-L-CD4-1F6 P (HIER) W
7 mL ready-to-use RTU-CD4-1F6 P (HIER)
Clone 4B12 was developed to allow conventional protocol where
endogenous peroxidase is blocked before primary antibody incubation to
produce superior staining on paraffin sections.
The CD4 molecule (T4) is a single chain transmembrane glycoprotein with a
molecular weight of 59 kD. The CD4 antigen is expressed on a T cell subset
(helper/inducer) representing 45 percent of peripheral blood lymphocytes
and at a lower level on monocytes. Most cases of cutaneous T cell
lymphoma, including mycosis fungoides, express the CD4 antigen and HTLV-
1 associated adult T cell leukemia/lymphoma is also generally CD4 positive.
Product Specific Information
Please note that the use of 1 percent or greater H2O2 to block endogenous
peroxidase has a detrimental effect on the epitope recognized by clone 1F6.
Therefore, it is recommended that endogenous peroxidase is blocked before
retrieval with 0.5 percent H2O2 /methanol for 10 minutes, otherwise staining
intensity may be reduced.
Refer to page 18 for the Bond ready-to-use format.
Human skin, mycosis fungoides: immunohistochemical staining for CD4 antigen using NCL-
CD4-1F6. Note membrane staining of infiltrating T lymphocytes. Paraffin section.
CD4 and CD8 Antibodies (duo pack)
Clone 1F6 and Clone 4B11
2 × 0.5 mL lyophilized NCL-CD4/CD8d P (HIER) W
For convenience, Leica Microsystems offer two antibodies in one pack.
Helper/inducer T cells (CD4 positive) and cytotoxic/suppressor T cells (CD8
positive) can be identified with the duo pack which supplies monoclonal
antibodies to both CD4 and CD8 antigens. Please note that clone 1F6
requires 0.5 percent H2O2 /methanol treatment for 10 minutes BEFORE
unmasking using EDTA to prevent any reduction in staining intensity. Please
also note that the use of 1 percent or greater H2O2 to block endogenous
peroxidase has a detrimental effect on the epitope recognized by clone 1F6.
CD5
Clone 4C7
1 mL, 0.1 mL lyophilized NCL-CD5-4C7 P (HIER) W
1 mL liquid NCL-L-CD5-4C7 P (HIER) W
7 mL ready-to-use RTU-CD5-4C7 P (HIER)
7 mL Bond ready-to-use PA0168 P (HIER)
Reference Range
CD5 antigen is reported to be expressed on 95 percent of thymocytes and 72
percent of peripheral blood lymphocytes. In lymph nodes, the main reactivity
is observed on T cells. CD5 antigen is also expressed by many T cell
leukemias, lymphomas, activated T cells and on a subset of B cells located
primarily in the mantle zones of normal lymph nodes. CD5 antigen
expression is also reported in T cell acute lymphocytic leukemias (T-ALL),
some B cell chronic lymphocytic leukemias (B-CLL) as well as B and T cell
lymphomas.
Refer to page 18 for the Bond ready-to-use format.
Human mantle cell lymphoma: immunohistochemical staining for CD5 antigen using
NCL-CD5-4C7. Note intense membrane staining of tumor cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
/71
Primary Antibodies