Labelling Review row-Online

Human small intestine: immunohistochemical staining for adenomatous polyposis coli protein
using NCL-APC. Note cytoplasmic staining of intestinal epithelial cells. Paraffin section.
Apolipoprotein J (Clusterin)
Clone 7D1
1 mL lyophilized NCL-CLUSTERIN F P (HIER)
See also Clusterin (Apolipoprotein J) on page 91.
Apoptosis Protease Activating Factor 1
Polyclonal
1 mL lyophilized NCL-APAF1 F P (HIER)
Apoptosis is one of a number of responses that may occur as a result of
signal transduction pathways in the cell. One identified mechanism for
initiating caspase activation requires the participation of mitochondria and
involves a 130 kD protein known as apoptosis protease activating factor-1
(Apaf-1). Apaf-1 is a cytosolic protein that remains in a latent state until
bound to cytochrome c (Apaf-2). Cytochrome c is commonly released from
the mitochondria during apoptosis induced by many, but probably not all cell
death stimuli. The resulting Apaf1/cytochrome c complex associates with
the zymogen form of caspase-9 (Apaf-3) in the presence of dATP or ATP,
promoting the autocatalytic activation of caspase-9. Once activated
caspase-9 can then cleave and activate procaspase-3 directly, resulting in a
cascade of additional caspase activation and apoptosis.
Aurora Kinase 2
Clone JLM28
1 mL liquid NCL-L-AK2 P (HIER) W
Aurora Kinase 1 and 2 encode cell cycle-regulated serine/threonine kinases
that are involved in microtubule spindle activities during mitosis and
meiosis. Aurora Kinase 2, also known as STK15, BTAK, ARK1 and AIK,
localizes to interphase and mitotic centrosomes and to the spindle poles. It
is degraded rapidly after G2/M phase release in mammalian cells. Aurora
Kinase 2 is reported to be expressed at high levels in testis and various
proliferating cell lines, including HeLa cells. Aurora Kinase 2 is regulated by
phosphorylation which is important both for its activity and stability. The
inhibition of its activity leads to the formation of a monopolar spindle
because its activity is necessary for centrosome separation. Aurora Kinase
2 overexpression leads to centrosome amplification, chromosome instability
and transformation in mammalian cells. Overexpression of both active and
inactive Aurora Kinase 2 can lead to polyploidy. This suggests that Aurora
Kinase 2 can behave as a dominant negative mutant and inhibit other aurora
kinases. When inactive kinase is expressed, however, the cells eventually
die and do not become immortalized, unlike with the active kinase.
HeLa cell line: immunohistochemical staining for Aurora Kinase using NCL-L-AK2. Note nuclear
staining of a proportion of cells. Paraffin section.
B Cell Marker (MB2)
Clone MB2
1 mL lyophilized NCL-MB2 FP
MB2 is a pan B cell marker that is expressed in all B cells except mature
plasma cells. It does not react with T cells. The antibody is weakly reactive
with endothelial cells and several types of epithelial cells. These include
epidermis (but excludes the squamous cell layer), epithelia of breast, lung,
pancreas, stomach, colon, bladder, fallopian tube and also hepatocytes and
stromal cells of the ovary. MB2 has been reported to react with an
uncharacterized cytoplasmic antigen found in both normal B cells and B cell
lymphomas.
Human tonsil: immunohistochemical staining for B lymphocytes using NCL-MB2. Note intense
cytoplasmic staining of normal B lymphocytes. Paraffin section.
B Cell Specific Octamer Binding
Protein-1 (BOB-1)
Clone TG14
1 mL, 0.1 mL liquid NCL-L-BOB-1 P (HIER)
7 mL Bond ready-to-use PA0558 P (HIER)
B cell specific octamer binding protein-1 (BOB-1), also known as OBF-1 and
OCA-B, is a lymphocyte specific transcriptional coactivator protein. It
interacts with OCT1 and OCT2 transcription factors and contributes to the
transcriptional activity of octamer motifs. BOB-1 has been reported to be
detectable in all B cell populations found in reactive lymphoid tissues. The
strongest expression being found in germinal center B cells and plasma
cells. The expression of BOB-1 in B cell tumors has been reported to be
variable.
Refer to page 15 for the Bond ready-to-use format.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies