Labelling Review row-Online

Human mesothelioma: immunohistochemical staining for calretinin using NCL-L-CALRET-566.
Note cytoplasmic staining of malignant cells. Paraffin section.
Carbonic Anhydrase IX
Clone TH22
1 mL, 0.1 mL liquid NCL-L-CAIX P (HIER) W
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of
carbon dioxide and water into carbonic acid, protons, and bicarbonate ions.
Originally named MN/G250, carbonic anhydrase IX (CAIX) is a cell surface
transmembrane protein, which is predominantly found in the gastrointestinal
tract and gall bladder. The glandular regions of normal colon are reported to
be negative, but in the case of adenocarcinoma, the glands are positive.
CAIX is also reported to be expressed in common epithelial tumors such as
carcinomas of the esophagus, lung, colon, kidney, cervix and non-small cell
lung carcinoma. In breast carcinomas, CAIX expression has been reported
to be associated with malignant tissue. Expression of CAIX is reported to be
absent in normal kidney, chromophobe carcinomas or oncocytomas,
however, it is specifically expressed in clear cell renal carcinomas.
Human stomach: immunohistochemical staining for carbonic anhydrase IX using NCL-L-CAIX.
Note intense membrane and cytoplasmic staining of the mucus secreting cells of the deep
glands. Paraffin section.
Carboxypeptidase M
Clone 1C2
1 mL lyophilized NCL-CPMm F P (HIER)
Carboxypeptidase M is a membrane bound glycoprotein of 62 kD. It is an
enzyme structurally, catalytically and immunologically distinct from
pancreatic carboxy-peptidase A and B, human plasma carboxypeptidase N
and carboxy-peptidase H. The functional role of carboxypeptidase M may be
to inactivate or modulate peptide hormones at local tissue sites before or
after their interaction with specific plasma membrane receptors.
Carboxypeptidase M is found on the placental microvilli, a site at which
materno-fetal exchange takes place. This site is rich in other peptidases
whose function is to inactivate deleterious peptides before crossing this
important barrier. Carboxypeptidase M is also found in peripheral nerves, at
different concentrations in various regions of the brain, in alveolar type 1
epithelial cells and alveolar macrophages.
Carcinoembryonic Antigen (CD66e)
Clone 12-140-10
1 mL lyophilized NCL-CEA-2 F P (Enzyme)
1 mL liquid NCL-L-CEA-2 F P (Enzyme)
7 mL ready-to-use RTU-CEA-2 F P (Enzyme)
Clone II-7
7 mL Bond ready-to-use PA0004 P (HIER)
Carcinoembryonic antigen (CEA) is a heterogeneous cell surface
glycoprotein produced by cells of fetal colon. Low levels are also found on
normal mucosal epithelia of the adult colon and a variety of other normal
tissues. CEA is encoded by the CEA gene that is located on chromosome 19.
It is a member of the CEA gene family, which in turn is a subfamily of the
immunoglobulin superfamily. Cell adhesion properties are now well
recognized for CEA. It is believed that the expression of this glycoprotein in
conjunction with other known adhesion molecules will influence the cellcell
interaction.
Refer to page 17 for the Bond ready-to-use format.
Caspase-2
Clone 10H2
1 mL lyophilized NCL-CASP-2 P (HIER)
Caspases are an extended family of cysteine proteases that play critical
roles in apoptosis. Caspases are synthesized as precursors known as procaspases
and these are converted into mature enzymes by apoptotic
signals. Caspase-2 is an early effector in the apoptotic cascade and
precedes the activation of caspase-3. It is generally found in the nucleus,
but pro-caspase-2 may also be detected in mitochondrial and cytosolic
fractions. Lymphoid organs display only weak caspase-2 expression,
located in sinusal histiocytes and thymic epithelial cells. Extra-lymphoid
caspase-2 reactivity is found in particular organs like the kidney. The
caspase-2/caspase-3 cascade may be inhibited by the bcl-2 oncoprotein.
Human testis: immunohistochemical staining for caspase-2 using NCL-CASP-2. Note intense
nuclear staining of a proportion of the spermatogonia in the seminiferous tubules. Paraffin
section.
Caspase-3 (CPP32)
Clone JHM62
1 mL, 0.1 mL lyophilized NCL-CPP32 P (HIER) W
See also CPP32 (Caspase-3) on page 93.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies