Labelling Review row-Online

Primary Antibodies
Western blot: analysis of human skeletal muscle showing detection of the calpain family of
proteins. Lane A, calpain 3 bands at 94 and 30 kD detected with NCL-CALP-2C4. Lane B,
calpains 1, 2 and 3 detected with NCL-CALP-11B3. Lane C, calpain 3 bands at 94 and
approximately 60 kD detected with NCL-CALP-12A2. Photograph supplied courtesy of Dr Louise
V B Anderson.
Calponin (Basic)
Clone 26A11
1 mL, 0.1 mL lyophilized NCL-CALPONIN-B F P (HIER) W
7 mL Bond ready-to-use PA0416 P (HIER)
Basic calponin (calponin-h1) is a 34 kD protein which exhibits a high degree
of homology to acidic and neutral calponins at its N-terminal region. It is an
actin, tropomyosin and calmodulin binding protein thought to be involved in
the regulation of smooth muscle contraction. The expression of basic
calponin is reported to be restricted to smooth muscle cells and is a marker
of the differentiated contractile phenotype of developing smooth muscle.
Vascular smooth muscle cells convert to a synthetic dedifferentiated
phenotype when this protein is lost and this is a key stage in both
atherosclerosis and restenosis of coronary arteries after balloon
angioplasty. It is thought that basic calponin exerts its effect via the cortical
actin cytoskeleton and therefore influences proliferation, the transformed
phenotype and the metastatic potential of tumor cells. Basic calponin mRNA
is expressed in smooth muscle of prostate, bowel and aorta whereas neutral
and acidic calponin mRNAs are expressed in non-smooth muscle tissues
such as heart, placenta, lung, kidney, pancreas, spleen, testis and ovary as
well as in smooth muscle-containing tissues.
Refer to page 16 for the Bond ready-to-use format.
Normal human appendix: immunohistochemical staining for basic calponin using
NCL-CALPONIN-B. Note cytoplasmic staining of smooth muscle. Paraffin section.
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Calretinin (5A5)
Clone 5A5
1 mL, 0.1 mL lyophilized NCL-CALRETININ P (HIER)
1 mL liquid NCL-L-CALRETININ P (HIER)
7 mL ready-to-use RTU-CALRETININ P (HIER)
Calretinin is an intracellular calcium-binding protein of 31.5 kD belonging to
the troponin C superfamily characterized by a structural motif described as
the EF-hand domain. Calcium is an important moderator of a number of vital
physiological processes, including neuronal excitability, axonal transport,
synthesis and release of some neurotransmitters, membrane permeability
and enzyme activity. Calretinin is found in the nervous system and thymus.
Calretinin can also be demonstrated in normal and neoplastic mesothelial
cells and has been reported to be a useful marker for the identification of
malignant mesotheliomas of epithelial type to differentiate these from
metastases of lung adenocarcinoma where antibodies to detect calretinin
are used in within a panel and interpretation together with clinical data is
undertaken by a qualified pathologist.
Human mesothelioma: immunohistochemical staining for calretinin using NCL-L-CALRETININ.
Note cytoplasmic staining of malignant cells. Paraffin section.
Calretinin (CAL6)
Clone CAL6
1 mL liquid NCL-L-CALRET-566 P (HIER) W
7 mL Bond ready-to-use PA0346 P (HIER)
Calretinin is a calcium-binding protein of 29 kD that is a member of the family
of so-called EF-hand proteins that also includes S-100 proteins. Calretinin is
reported to be abundantly expressed in neurons. Outside the nervous
system, calretinin is reported to be expressed in a range of cell types
including mesothelial cells, steroid producing cell, (eg adrenal cortical cells,
Leydig cells, ovarian theca interna cells as well as Sertoli cells, some
neuroendocrine cells, eccrine sweat glands) and other cell types. The
presence of calretinin is reported to be a useful marker primarily for
differentiating malignant mesothelioma from carcinomas. Other reports
indicate the detection of calretinin is helpful for the differential
characterization of ovarian stroma tumors. Calretinin-positive cells have
also been reported in the convoluted tubules of kidney with some
antibodies.
Refer to page 17 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range