Labelling Review row-Online

Glutathione S-Transferase (GST)
Antibodies
Clone 38H11
1 mL lyophilized Glutathione S-Transferase (alpha)
NCL-GSTal-436 P
Clone 10H6
1 mL lyophilized Glutathione S-Transferase (mu)
NCL-GSTmu-437 P
Clone LW29
1 mL, 0.1 mL lyophilized Glutathione S-Transferase (pi)
NCL-GSTpi-438 FP
The glutathione S-transferases (GSTs) are a multigene family of isoenzymes
which catalyze the conjugation of glutathione to electrophilic substrates.
These enzymes are involved in the detoxification of both endogenous and
exogenous electrophiles which can react with cellular components such as
DNA. The modification of DNA by reactive compounds can initiate carcinogenesis
and the GSTs are believed to play a role in neutralizing carcinogens.
The cytosolic GST isoenzymes have been classified into four evolutionary
classes; alpha, mu, pi and theta. These isoenzymes are reported to be singly
or multi-expressed in a variety of normal tissues, including stomach, bowel,
brain, heart, liver, pancreas, breast, kidney and skin at differing levels. In
gastric cancers, the levels of GSTalpha and pi are reported to differ from
normal gastric tissue with GSTalpha showing decreased levels and GSTpi
increased levels. High levels of GSTpi are reported in some breast cancer
cells and have been associated with chemotherapeutic agents. Likewise, in
human lung squamous cell carcinomas, high levels of GSTpi are reported to
be associated with resistance to the drug cisplatin. The expression of
GSTmu has been reported to be high in acute myeloid leukemias. GSTmu is
also known to play a role in detoxification of epoxides released from
cigarette smoke.
Human testis: immunohistochemical staining for glutathione S-transferase mu using
NCL-GSTmu-437. Note cytoplasmic staining of sertoli cells. Paraffin section.
Gonadotropin-Releasing Hormone
Receptor
Clone A9E4
1 mL lyophilized NCL-GnRHR P (HIER)
Gonadotropin-releasing decapeptide hormone is the key mediator in the
integration of the neural and endocrine systems. It regulates the reproductive
cycle in both sexes. Gonadotropin-releasing hormone (GnRH) stimulates
the gonadotrophs of the anterior pituitary to secrete luteinizing hormone as
well as follicle stimulating hormone via specific receptors. The presence of
high-affinity binding sites for GnRH has been reported in luteal and
granulosa cells as well as in ovarian cell membrane preparations. These
receptors have similar binding characteristics to those of GnRH receptors in
the anterior pituitary.
Product Specific Information
NCL-GnRHR is specific for the extracellular domain of the GnRH receptor.
GPIb (CD42b)
Clone MM2/174
1 mL lyophilized NCL-CD42b F P (HIER)
See also CD42b (GPIb) on page 79.
GPIIb/IIIa (CD41)
Clone M148
1 mL lyophilized NCL-CD41 F
See also CD41 (GPIIb/IIIa) on page 79.
GPIIIa (CD61)
Clone 2f2
1 mL, 0.1 mL lyophilized NCL-CD61-308 F P (HIER)
7 mL Bond ready-to-use PA0308 P (HIER)
See also CD61 (GPIIIa) on page 82.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies