Labelling Review row-Online

Primary Antibodies
c-MET (Hepatocyte Growth Factor
Receptor)
Clone 8F11
1 mL, 0.1 mL lyophilized NCL-cMET F P (HIER)
The c-MET gene encodes a transmembrane tyrosine kinase identified as the
receptor for a polypeptide known as hepatocyte growth factor (HGF). HGF
has been shown to exert a pleiotropic activity on several cell types mainly of
epithelial origin. It is a powerful mitogen for hepatocytes and also stimulates
the growth of other cell types including kidney tubular cells, keratinocytes
and endothelial cells. Other cell types known to express c-MET include
hepatocytes, microglial cells in white matter and astrocytes.
c-myc Oncoprotein
Clone 9E11
1 mL, 0.1 mL lyophilized NCL-cMYC FP
The c-myc oncogene is the human cellular homolog of the avian v-myc gene
found in several leukemogenic retroviruses. c-myc is a nuclear phosphoprotein,
which has DNA-binding activity and is implicated in the control of
normal proliferation and differentiation. Expression of c-myc in
untransformed cells is growth factor dependent and essential for
progression through the cell cycle. c-myc is expressed during proliferation
in a wide variety of adult tissues and at all stages of embryonic
development.
Product Specific Information
Enzyme pretreatment may enhance staining in some cases.
Collagen Type II
Polyclonal
1 mL lyophilized NCL-COLL-IIp P (Enzyme)
Collagen type II is the structural protein predominantly found throughout the
cartilage matrix and is also found in very small amounts in the eye. The
fibrils formed by this protein are usually thinner and more delicate than
collagen type I fibrils.
Product Specific Information
NCL-COLL-IIp reacts with type II collagen and does not cross-react with
collagen types I, III, IV, V, VI, other human serum proteins or non-collagenous
extracellular associated proteins.
Human chondrosarcoma: immunohistochemical staining for collagen type II using NCL-COLL-
IIp. Note diffuse staining of chondroid tissue. Paraffin section.
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Collagen Type IV
Clone PHM-12
1 mL lyophilized NCL-COLL-IV F P (HIER+Enzyme)
In kidney, collagen IV is expressed in glomerular and tubular basement
membranes and also mesangial cells and the matrix within glomeruli, the
basal lamina of capillaries as well as basement membrane structures in
many organs.
Product Specific Information
The heat induced epitope retrieval (HIER) technique followed immediately by
30 seconds of enzyme digestion produces optimal staining with this antibody.
NCL-COLL-IV recognizes collagen type IV, which is a major constituent of
basement membranes.
Normal human kidney: immunohistochemical staining for collagen type IV using NCL-COLL-IV.
Note staining of tubular basement membranes, mesangial cells and the glomerular matrix.
Paraffin section.
Collagen Type VII
Clone LH7.2
1 mL lyophilized NCL-COLL-VII F
Collagen type VII is a basement membrane component which is the major
protein in the anchoring fibrils projecting from the lamina densa into the
subjacent connective tissue. Collagen type VII has been reported to be
detected in the basal lamina of stratified epithelia such as epidermis, oral,
oesophageal and cervical epithelium and urothelium of the bladder. Those
epithelia which are composed of different cell types eg sweat gland
epithelium or breast epithelium which are made up of myoepithelial cells
next to glandular cells, possess a type VII collagen-containing basement
membrane. Basement membranes play an important role in tumor
progression. In normal breast tissue, benign breast lesions and in situ
malignancies, the basement membrane always surrounds ducts and tubules
whereas in invasive breast carcinomas it is often absent. Collagen type VII is
reported to be a defective membrane component in the condition Recessive
Dystrophic Epidermolysis Bullosa (RDEB).
Human skin: immunohistochemical staining for collagen type VII using NCL-COLL-VII.
Note staining of the basal lamina of the stratified epithelium. Frozen section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.