Labelling Review row-Online

Primary Antibodies
CD57
Clone NK-1
1 mL, 0.1 mL lyophilized NCL-NK1 FP
7 mL ready-to-use RTU-NK1 FP
7 mL Bond ready-to-use PA0443 P (HIER)
The CD57 glycoprotein, also known as HNK-1, has a molecular weight of 110
kD. It is found on a subset of mononuclear cells with natural killer activity
and on neuroectodermal cells expressing myelin-associated glycoprotein.
Many cells which co-express CD57 and CD8 proteins are a subset of
suppressor/cytotoxic T cells. These cells play a role in the rejection of grafts
in acute graft versus host disease. The CD57 molecule is not expressed on
erythrocytes or platelets.
Product Specific Information
Enzyme pretreatment may enhance staining in some cases.
Refer to page 23 for the Bond ready-to-use format.
Human lymph node: immunohistochemical staining for CD57 antigen using NCL-NK1.
Note staining of CD57 positive T lymphocytes. Paraffin section.
CD61 (GPIIIa)
Clone 2f2
1 mL, 0.1 mL lyophilized NCL-CD61-308 F P (HIER)
7 mL Bond ready-to-use PA0308 P (HIER)
The CD61 antigen, also known as GPIIIa, is a glycoprotein of 105 kD found on
platelets, monocytes, endothelial cells, smooth muscle cells, B cells,
macrophages, mast cells and fibroblasts. CD61 antigen plays a role in
platelet aggregation and also as a receptor for fibrinogen, fibronectin, von
Willebrand factor and vitronectin. Individuals with Glanzmann's thrombasthenia
are reported to express little or no CD61 antigen. CD61 antigen is
also reported to be expressed in most cases of megakaryocytic leukemias.
Refer to page 23 for the Bond ready-to-use format.
Human tonsil: immunohistochemical staining for CD61 antigen (GPIIIa) using NCL-CD61-308.
Note staining of platelets within the blood vessels. Paraffin section.
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CD62L (L-selectin)
Clone 9H6
1 mL lyophilized NCL-CD62L-489 P (HIER)
See also L-selectin (CD62L) on page 128.
CD62P (P-selectin)
Clone C34
1 mL lyophilized NCL-CD62P-367 P (HIER)
See also P-selectin (CD62P) on page 155.
CD63 (Melanoma Marker)
Clone NKI/C3
1 mL, 0.1 mL lyophilized NCL-CD63 FP
CD63 antigen is a member of the TM4 superfamily with its structure
consisting of four transmembrane regions, short cytoplasmic N and Ctermini
and two extracellular regions. CD63 antigen is widely distributed on
the surface and interior of both hematopoietic and non-hematopoietic cells
such as most sweat glands, islets of Langerhans, pituitary, pancreas,
peribronchial glands, Paneth cells and prostate glands. It is reported to be
strongly expressed on monocytes, macrophages and activated platelets and
weakly expressed on lymphocytes and granulocytes. CD63 antigen
associates non-covalently with CD9, CD81 and the integrins VLA-3, VLA-4
and VLA-6. It is reported that CD63 antigen may play a role as a tumor
suppressor gene as its expression in human melanoma cells reduces tumor
spread and metastasis.
CD66a (CEACAM1)
Clone 29H2
1 mL lyophilized NCL-CD66a P (HIER)
CD66a antigen, also known as biliary glycoprotein (BGP), is a member of the
carcinoembryonic antigen (CEA) family and of the immunoglobulin superfamily.
CD66a, CD66b, CD66c and CD66d are known to be expressed by
hematopoietic cells. CD66a antigen is also expressed in epithelia, in certain
endothelia and in cells of the myeloid lineage. In liver, expression occurs in
hepatocytes along the bile canaliculi. Apical membranous expression of the
antigen is reported to occur in enterocytes, superficial absorptive cells of
the colon, epithelia of oesophageal and Brunner's glands, bile ducts and gall
bladder, pancreatic ducts, proximal tubules of the kidney, prostate,
endometrium and mammary ducts. Selective expression in endothelia is
reported to occur in glomeruli and vasa recta of the kidney, small placental
vessels, adrenal sinusoids, endometrium and prostate. Among the cells of
the myeloid lineage, granulocytes and myelocytes express CD66a antigen.
The expression of CD66a antigen has also been reported to be downregulated
in breast, endometrial, colorectal and prostate cancers. However,
in one specific study of breast cancer, CD66a antigen was found not to be
downregulated.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.