Labelling Review row-Online

Primary Antibodies
LFA-2 (CD2)
Clone AB75
1 mL, 0.1 mL lyophilized NCL-CD2-271 P (HIER)
1 mL liquid NCL-L-CD2-271 P (HIER)
7 mL ready-to-use RTU-CD2-271 P (HIER)
See also CD2 (LFA-2) on page 70.
Linker for Activation of T Cells
Clone 3.8
1 mL liquid NCL-L-LAT F P (HIER)
7 mL ready-to-use RTU-LAT F P (HIER)
Stimulation of the T cell antigen receptor (TCR) results in the activation of
several protein tyrosine kinases (PTKs) associated with the TCR. These
activated PTKs phosphorylated tyrosine residues on multiple protein
substrates. This phosphorylation results in the activation of enzymes such
as phospholipase C gamma or creates sites of binding for proteins involved
in the activation cascade. Linker for activation of T cells (LAT) is an integral
membrane protein (36 to 38 kD) which plays an important role in linking
engagement of the TCR to the biochemical events of T cell activation. LAT is
a substrate of activated ZAP-70 and Syk PTKs. It binds following tyrosine
phosphorylation, Grb2, PLC-gamma1 and other critical signalling molecules
recruiting them to the plasma membrane. This has the effect of enhancing
the phosphorylation of tyrosine residues required for enzymatic activation
and promoting the formation of protein complexes. LAT mRNA is found in NK
cells and mast cells. LAT protein has been reported to be detected in thymus
and peripheral lymphoid tissues such as T cell areas in lymph nodes and
spleen. In the small intestine, intra-epithelial T cells also express LAT, and in
bone marrow, LAT is expressed by T lymphocytes in interstitial spaces and
also by platelets and megakaryocytes. LAT is reported not to be expressed
in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and
dendritic cells.
Human tonsil: immunohistochemical staining for LAT protein using NCL-L-LAT. Note intense
membrane staining of T lymphocytes. Paraffin section.
LMP-1 (Epstein-Barr virus)
CS1, CS2, CS3, Clone CS4
1 mL, 0.1 mL lyophilized NCL-EBV-CS1-4 F P (Enzyme)
See also Epstein-Barr virus (LMP-1) on page 106.
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L-selectin (CD62L)
Clone 9H6
1 mL lyophilized NCL-CD62L-489 P (HIER)
The CD62L antigen is also known as Leu-8, TQ1, LAM1, MEL-14 antigen,
lymph node homing antigen and L-selectin. It mediates the tethering and
rolling of leukocytes on endothelial surfaces and also contributes to the
recruitment of leukocytes from the blood to areas of inflammation. CD62L
antigen is also important for the homing of naive lymphocytes to peripheral
lymph nodes and Peyer's patches and can also mediate neutrophil to
neutrophil interactions via the recognition of CD162 antigen. CD62L antigen
is reported to be expressed on the surface of mantle zone B lymphocytes in
different lymphoid sites but is absent on germinal center B cells. It is also
expressed on a proportion of T cells in peripheral lymph nodes, mucosal
lymphoid sites and spleen. Non-lymphocytic staining has been reported on
Langerhans cells, follicular dendritic cells in tonsil, neutrophils, monocytes
and on macrophages in the thymus. Ligands for CD62L are expressed not
only in specific vascular endothelium in lymph nodes and Peyer's patches
but also in extravascular tissues such as brain white matter, the choroid
plexus and in kidney distal tubuli.
Human tonsil: immunohistochemical staining for CD62L antigen (L-selectin) using
NCL-CD62L-489. Note membrane staining of lymphocytes. Paraffin section.
Lung Resistance-Related Protein (110 kD)
Clone 9D6
1 mL lyophilized NCL-LRRP P (HIER) W
Multidrug-resistant cancer cell lines are reported to frequently overexpress
the 100 kD lung resistance protein (LRP) also known as lung resistancerelated
protein (LRRP). The overexpression of LRRP in acute myeloid
leukemias, multiple and ovarian carcinomas has been reported. LRRP
functions as a major vault transporter protein where vaults are multi-subunit
structures which may be involved in nucleocytoplasmic transport. LRRP is
overexpressed in P-glycoprotein negative multidrug-resistant tumor cell
lines of different histogenetic origins and show an ATP-dependent drug
accumulation effect. LRRP is also reported to be expressed in normal
tissues, with expression being highest in epithelial cells with secretory and
excretory functions such as bronchial cells and intestinal epithelial cells.
Normal human testes: immunohistochemical staining for lung resistance-related protein
(110 kD) using NCL-LRRP. Note cytoplasmic staining of cells within the seminiferous tubules.
Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.