Labelling Review row-Online

Primary Antibodies
Terminal Deoxynucleotidyl Transferase
Clone SEN28
1 mL, 0.1 mL lyophilized NCL-TdT-339 P (HIER) W
1 mL liquid NCL-L-TdT-339 P (HIER) W
7 mL ready-to-use RTU-TdT-339 P (HIER)
7 mL Bond ready-to-use PA0339 P (HIER)
Terminal deoxynucleotidyl transferase (TdT) is a DNA polymerase of 58 kD
located in the cell nucleus which catalyzes the polymerization of
deoxynucleotides at the 3' hydroxyl ends of oligo or polydeoxynucleotide
initiators and functions without a template. TdT is reported to be expressed
in primitive T and B lymphocytes of the normal thymus and bone marrow.
The identification of TdT-positive cell populations in primary and secondary
lymphoid organs during maturation of the immune system is one area of
interest but it is the reported occurrence of high levels of enzyme activity in
white blood cells and bone marrow in certain leukemias which is of
particular interest. Early and precise differentiation of lymphoblastic
lymphoma is crucial. TdT is reported to be expressed in lymphoblastic
lymphomas and leukemias. The determination of TdT expression is reported
to be most valuable when it is difficult to differentiate histologically between
lymphoblastic lymphoma and Burkitt's lymphoma.
Refer to page 42 for the Bond ready-to-use format.
Human lymphoblastic lymphoma: immunohistochemical staining for terminal deoxynucleotidyl
transferase using NCL-TdT-339. Note intense nuclear staining of tumor cells. Paraffin section.
Thrombomodulin (CD141)
Clone 15C8
1 mL, 0.1 mL lyophilized NCL-CD141 F P (HIER)
Thrombomodulin is a transmembrane glycoprotein of 75 kD which can
accelerate the activation of protein C. Activated protein C functions as an
anticoagulant by combining with protein S to inactivate factors Va and VIIIa
of the blood coagulation pathway and by binding thrombin. Several factors
regulate thrombomodulin expression. Downregulation of thrombomodulin
may be induced by the cytokine interleukin-1, tumor necrosis factor and
endotoxin. Agents which increase cyclic AMP such as forskolin may
upregulate thrombomodulin activity in endothelial cells. Thrombomodulin
has been identified within a number of normal tissues. These include the
lining cells of arteries, veins, capillaries and the lymphatics as well as
mesothelial cells, meningeal lining cells, synovial cells, syncytiotrophoblasts,
megakaryocytes and platelets. It is reported that in oral
squamous cell carcinomas, the reduction of thrombomodulin expression
may play a role in metastasis. Thrombomodulin protein is reported to be
absent from most pulmonary adenocarcinomas but is expressed in
malignant pleural mesotheliomas, vascular tumors and choriocarcinomas.
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Reference Range
Thymus and Activation-Regulated
Chemokine
Clone 6SN
1 mL lyophilized NCL-TARC P (HIER)
The attraction of leukocytes to sites of inflammation is a process controlled
by chemokines. Chemokines are a group of proteins of 8 to 10 kD that are
divided into four families based on their first two cysteine residues in the
conserved motif. Thymus and activation-regulated chemokine (TARC)
functions as a selective chemoattractant for T cells which express a class of
receptors binding TARC with high affinity and specificity. TARC has been
identified as the specific ligand for the CC chemokine receptor 4 (CCR4)
which is preferentially expressed at high levels in activated T helper 2 (Th2)
cells. It is reported to be constitutively expressed in the dendritic cells of the
thymus and transiently in activated peripheral blood mononuclear cells. It is
present in the cell cytoplasm with frequent paranuclear condensation.
Reports indicate that TARC is overexpressed in nodular sclerosis (NS) and
mixed cellularity (MC) classical Hodgkin's disease but not in non-Hodgkin's
lymphomas, anaplastic large cell lymphomas and large B cell lymphomas
with CD30 positivity. Reed Sternberg cells of NS and MC Hodgkin's
lymphomas show high levels of TARC protein. Furthermore, in classical
Hodgkin's disease, TARC's ability to attract activated T cells by strong
association to the chemokine receptor, CCR4, may explain the characteristic
T cell infiltrate. It has also been suggested that TARC may also play a role in
platelet activation, via CCR4, seen in Th2-associated diseases such as
asthma and atopic dermatitis.
Human cervical lymph node, Hodgkin's disease: immunohistochemical staining for thymus and
activation-regulated chemokine (TARC) using NCL-TARC. Note characteristic staining of Reed
Sternberg cells. Paraffin section.
Thyroglobulin
Clone 1D4
1 mL liquid NCL-L-THY FP
7 mL Bond ready-to-use PA0025 P
Thyroglobulin is a heavily glycosylated protein of 670kD composed of two
identical subunits and is synthesised by the follicular epithelial cells of the
thyroid. Thyroglobulin provides iodination sites for the formation of the
thyroid hormones.
Refer to page 42 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.