Labelling Review row-Online

Primary Antibodies
Tyrosinase-Related Protein-1
Clone G3E6
1 mL lyophilized NCL-TRP-1 P (HIER)
Tyrosinase-related protein-1 (TRP-1) is a member of a family of proteins
which are involved in melanin biosynthesis. The catalytic function of TRP-1
has not been fully resolved but the enzyme appears to be important in the
oxidation of 5,6-dihydroxyindole-2-carboxylic acid to form a high molecular
weight pigmented biopolymer. In mammals, there are two basic types of
melanin, the brown-black eumelanin and the reddish-yellow phaeomelanin.
The concentrations of each are variable and are not related to skin type. In
skin exposed to suberythemal doses of UVB, an increase in the number of
melanocytes expressing TRP-1 and TRP-2 is reported with no increase in the
number of tyrosinase-expressing melanocytes. In normal, untreated skin the
number of melanocytes that express either TRP-1, TRP-2 or tyrosinase are
similar irrespective of skin type. TRP-1 is also reported to be expressed in
more than 50 percent of choroidal melanocytes in the adult eye.
Human malignant melanoma: immunohistochemical staining for tyrosinase-related protein-1
using NCL-TRP-1. Note cytoplasmic staining of tumor cells. Paraffin section.
Tyrosine Hydroxylase
Clone 1B5
1 mL, 0.1 mL lyophilized NCL-TH P (HIER) W
Tyrosine hydroxylase is the first enzyme in catecholamine (CA) biosynthesis
and catalyzes the conversion of L-tyrosine to L-DOPA. Tyrosine hydroxylase
is reported to be expressed in all CA neurons. Despite the abundant data
about the distribution of catecholaminergic neurons in a wide variety of
species, data on their distribution in the human brain is less comprehensive.
However, one such study has reported that tyrosine hydroxylase products in
the substantia nigra were restricted to neural bodies, axons and dendrites.
These in turn were restricted to the third decade of life and their number
increased in this location with age. This finding may be related to ageing of
melanin-pigmented neurons.
Product Specific Information
NCL-TH is reactive with tyrosine hydroxylase in human, mouse and rat brain
tissue.
Human midbrain: immunohistochemical staining of tyrosine hydroxylase enzyme using
NCL-TH. Note cytoplasmic staining of catecholaminergic cells and their processes.
Paraffin section (Peroxidase substrate: nickel DAB, Counterstain: eosin).
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Ubiquitin
Clone FPM1
1 mL lyophilized NCL-UBIQm P
Polyclonal
1 mL lyophilized NCL-UBIQ FP
Ubiquitin, a small protein consisting of 76 amino acids, has been reported to
be found in all eukaryotic cells studied. It is one of the most conserved
proteins known. Ubiquitin is required for ATP-dependent, non-lysosomal
intracellular protein degradation, which eliminates most intracellular
defective proteins as well as normal proteins with a rapid turnover.
Degradation involves covalent binding of ubiquitin to the protein to be
degraded and it is believed that in this way ubiquitin acts to label the protein
for disposal by intracellular proteases. The most abundant ubiquitin-protein
conjugate, however, is ubiquitin-histone H2A. This conjugate is not
degraded. Since such ubiquitinated histones are present primarily in
actively transcribed chromosomal regions, ubiquitin may play a role in
regulation of gene expression.
Utrophin
Clone DRP3/20C5
2.5 mL, 1 mL lyophilized NCL-DRP2 FE
Utrophin, located on chromosome 6, is a ubiquitously expressed homologue
of dystrophin and is known as dystrophin-related protein. In normal muscle,
utrophin is restricted to neuromuscular junctions. However, in dystrohindeficient
muscle, utrophin expression is reported to be upregulated and
appears around the periphery of most fibres. Utrophin has a role as a cell
anchoring molecule. The amino terminal region of utrophin binds to the actin
cytoskeleton, acting as an intracellular anchor whereas the carboxyl
terminal regions bind to a group of proteins anchored in the cell membrane.
Varicella-zoster virus
Clone C90.2.8
1 mL lyophilized Varicella-zoster virus NCL-VZV PI
Varicella-zoster virus is a member of the alphaherpesvirinae. It is responsible
for two ubiquitous diseases: varicella (chickenpox), an exanthem of
childhood, and herpes zoster (shingles), a disabling disease of the elderly
and immunocompromised individuals.
Product Specific Information
NCL-VZV is specific for Varicella-zoster virus and does not cross-react with
Respiratory syncytial virus, Parainfluenza virus types 1, 2, 3 and 4b,
Adenovirus, Herpes simplex virus types 1 and 2, Influenza virus types A and
B, Mumps virus, Measles virus, echovirus 19, Coxsackie B4 virus and
Poliovirus types 1, 2 and 3.
Human skin: immunohistochemical staining for Varicella-zoster virus using NCL-VZV.
Note staining of infected cells within a vesicle. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.