Labelling Review row-Online

Thyroid Peroxidase
Clone AC25
1 mL liquid NCL-L-TPO P (HIER)
Thyroid Peroxidase gene expression is under the regulation of thyroid
stimulating hormone (TSH). In normal thyroid, expression of Thyroid
Peroxidase (TPO) described immunohistochemically is reported to produce
a diffuse, fine, granular cytoplasmic stain in all follicular cells. Some studies
have shown qualitative, as well as quantitative differences in thyroid
peroxidase expression in thyroid cancer compared to normal tissue.
Product Specific Information
NCL-L-TPO stains optimally when used in TBS-based wash buffer and diluent
systems.
Thyroid, Grave’s disease: immunohistochemical staining for thyroid peroxidase using
NCL-L-TPO. Note intense cytoplasmic staining of thyroid epithelial cells. Paraffin section.
Thyroid Stimulating Hormone
Clone QB2/6
1 mL lyophilized NCL-TSH F P (Enzyme)
7 mL Bond ready-to-use PA0776 P (Enzyme)
Thyroid stimulating hormone (TSH) is a pituitary hormone of 28 kD which
stimulates thyroid growth and production of thyroid hormones. TSH is
reported to be expressed in thyrotrophic cells of the pituitary and pituitary
adenomas.
Refer to page 42 for the Bond ready-to-use format.
Normal human pituitary gland: immunohistochemical staining for thyroid stimulating hormone
using NCL-TSH. Note cytoplasmic staining of a proportion of anterior pituitary cells. Paraffin
section.
Thyroid Stimulating Hormone Receptor
Clone 4C1/E1/G8
1 mL lyophilized NCL-TSH-R2 FP
The thyroid stimulating hormone receptor (TSHR) is an important molecule
for the control of growth and function of normal thyroid and in humans it is
frequently an autoimmune target. In normal human thyroid tissues, TSHR is
reported to be detected exclusively along the basal cell surface of the
follicular cells with no expression observed in apical and lateral cell
surfaces. Expression is absent in squamous cell metaplasia of papillary
carcinoma, anaplastic carcinoma and medullary carcinoma. TSHR is
reported to be preserved essentially in the basal cell surface of the thyroid
follicular cells in neoplastic conditions and is increased in Graves' disease.
Normal human thyroid gland: immunohistochemical staining for thyroid stimulating hormone
receptor using NCL-TSH-R2. Note cytoplasmic staining of thyroid epithelial cells. Paraffin
section.
Thyroid Transcription Factor-1
Clone SPT24
1 mL, 0.1 mL lyophilized NCL-TTF-1 F P (HIER) W
1 mL liquid NCL-L-TTF-1 F P (HIER) W
7 mL Bond ready-to-use PA0364 P (HIER)
Reference Range
Thyroid transcription factor-1 (TTF-1) is a member of the homeodomain
transcription factor family and plays a role in regulating genes expressed
within the thyroid, lung and brain. These include thyroglobulin, thyroid
peroxidase, Clara cell secretory protein and surfactant proteins. Human
TTF-1 (38 kD) is a single polypeptide of 371 amino acids sharing 98 percent
homology with the equivalent rat and mouse proteins. TTF-1 functions by
binding to specific recognition sites in a manner that may be regulated by
both the redox and phosphorylation status of the protein. In addition to its
role as a tissue-specific transcriptional activator in adult organs, TTF-1 may
also function in organogenesis. Gene targeting studies have shown TTF-1 to
be essential for the proper development of the thyroid and lungs and
abnormal expression may underline a number of congenital abnormalities.
TTF-1 has been reported to be expressed in greater than 90 percent of
pulmonary small cell carcinomas and in 75 percent of pulmonary non-small
cell carcinomas but it is not expressed in typical pulmonary carcinoids. TTF-
1 is also reported to be expressed in papillary, follicular carcinomas and
goitre, a non-malignant swelling of the thyroid, but not in anaplastic thyroid
carcinomas.
Refer to page 42 for the Bond ready-to-use format.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies