Labelling Review row-Online

Primary Antibodies
CD1a
Clone MTB1
1 mL, 0.1 mL lyophilized NCL-CD1a-235 F P (HIER)
1 mL liquid NCL-L-CD1a-235 F P (HIER)
7 mL ready-to-use RTU-CD1a-235 F P (HIER)
7 mL Bond ready-to-use PA0235 P (HIER)
Clone JPM30
1 mL, 0.1 mL lyophilized NCL-CD1a-220 F P (HIER)
CD1a is a protein of 43 to 49 kD expressed on dendritic cells and cortical
thymocytes. CD1a antigen expression has been shown to be useful in
differentiating Langerhans cells, powerful antigen presenting cells present
in skin and epithelia, from interdigitating cells. Immunohistochemical studies
for CD1a antigen have reported a reduction in epidermal Langerhans cells in
graft versus host disease and the participation of CD1a antigen-positive
dendritic cells in atherosclerotic lesion formation and asthmatic
inflammation.
Product Specific Information
Clone MTB1 detects cortical thymocytes, Langerhans cells in epidermis,
interdigitating cells of dermis and interdigitating cells of stratified squamous
epithelium of tonsil. Clone MTB1 may also detect small focal groups of
lymphocytes outside the germinal centers of tonsil indicating a crossreaction
with CD1b antigen. Clone JPM30 detects cortical thymocytes,
Langerhans cells in epidermis, interdigitating cells of dermis, interdigitating
cells of stratified squamous epithelium of tonsil but in addition it stains sweat
gland ducts in the dermis and epithelial cells of small intestine indicative of
cross-reactivity with CD1d antigen.
Refer to page 17 for the Bond ready-to-use format.
Normal human skin: immunohistochemical staining for CD1a antigen using NCL-CD1a-235.
Note intense membrane staining of Langerhans cells. Paraffin section.
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Reference Range
CD2 (LFA-2)
Clone AB75
1 mL, 0.1 mL lyophilized NCL-CD2-271 P (HIER)
1 mL liquid NCL-L-CD2-271 P (HIER)
7 mL ready-to-use RTU-CD2-271 P (HIER)
Clone 11F11
7 mL Bond ready-to-use PA0271 P (HIER)
The CD2 antigen (LFA-2) is a monomeric 45 to 58 kD glycoprotein. It is an
accessory molecule important in mediating the adhesion of activated T cells
and thymocytes with antigen-presenting cells and target cells.
Refer to page 17 for the Bond ready-to-use format.
Human small intestine, T cell lymphoma: immunohistochemical staining for CD2 antigen (LFA-2)
using NCL-CD2-271. Note intense membrane staining of T lymphocytes. Paraffin section.
CD3
Clone LN10
1 mL, 0.1 mL liquid NCL-L-CD3-565 P (HIER)
7 mL Bond ready-to-use PA0553 P (HIER)
Clone PS1
1 mL, 0.1 mL lyophilized NCL-CD3-PS1 P (HIER) W
1 mL liquid NCL-L-CD3-PS1 P (HIER) W
7 mL ready-to-use RTU-CD3-PS1 P (HIER)
Clone UCHT1
1 mL lyophilized NCL-CD3 FC
Clone LN10 was developed to produce superior staining with PBS based
buffers compared to clone PS1 on paraffin sections.
The CD3 molecule consists of five different polypeptide chains with
molecular weights ranging from 16 to 28 kD. The CD3 antigen is first
detected in early thymocytes and its appearance probably represents one of
the earliest signs of commitment to the T cell lineage.
Product Specific Information
Clone PS1 is specific for the non-glycosylated epsilon chain of the human CD3
molecule (Chetty R and Gatter K. Journal of Pathology. 173: 303-307 (1994)).
Clone LN10, our newest clone, is also specific for the non-glycosylated
epsilon chain of the human CD3 molecule. Clones LN10, PS1, and UCHT1
recognize T cells in thymus, bone marrow, peripheral lymphoid tissue and
blood and are all pan T cell markers.
Refer to page 18 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range