Labelling Review row-Online

EZH2 (Enhancer of Zeste Homolog 2
(Drosophila))
Clone 6A10
1 mL, 0.1 mL liquid NCL-L-EZH2 P (HIER) W
Polycomb-group proteins (PcG) such as EZH2 (Enhancer of Zeste Homolog 2
(Drosophila)) form multimeric gene repressing complexes involved in axial
patterning, hematopoiesis and cell cycle regulation. PcG proteins ensure
correct embryonic development by expressing homeobox genes as well as
contributing to the regulation of lymphopoiesis.
Product Specific Information
NCL-L-EZH2 stains optimally when used in TBS-based wash buffer and
diluent systems.
Diffuse large B cell lymphoma: immunohistochemical staining for EZH2 antigen using
NCL-L-EZH2. Note nuclear staining of malignant cells. Paraffin section.
Factor VIII-Related Antigen
(von Willebrand Factor)
Clone 36B11
1 mL, 0.1 mL lyophilized NCL-vWF F P (HIER)
1 mL liquid NCL-L-vWF F P (HIER)
7 mL Bond ready-to-use PA0400 F P (HIER)
See also Human von Willebrand Factor (Factor VIII-related antigen) on page
123.
Factor XIIIa (Blood Coagulation
Factor XIIIa)
Clone E980.1
1 mL, 0.1 mL lyophilized NCL-FXIIIa P (HIER)
7 mL Bond ready-to-use PA0449 P (HIER)
Factor XIIIa also known as fibrinoligase and fibrin-stabilizing factor, is the
last enzyme generated in the blood coagulation cascade. It is a Ca 2+ -
dependent transglutaminase or transamidating enzyme which forms
intermolecular gamma-glutamyl-epsilon-lysine crosslinks between fibrin
molecules resulting in the mechanical stabilization of the fibrin clot and its
resistance to proteolysis. Factor XIIIa may also function to stabilize cell
surface molecules and membranes. These Ca2+ -dependent transglutaminases
with thiol active centers are widespread in animal tissues and
have been associated with cell proliferation, embryonic development and
growth through the proliferation of mammary stroma and epithelial
elements. Normal mammary stroma, like most collagenous connective
tissue contains resident populations of CD34 positive dendritic interstitial
cells and scattered factor XIIIa positive collagen-associated dendrophages.
Factor XIIIa has been examined to determine its expression in normal and
inflamed skin. Factor XIIIa positive cells in human skin represent a specific
population of bone marrow dermal dendritic cells, distinct from Langerhans
cells which share some features common to mononuclear phagocytes. In
benign skin conditions such as inflammatory dermatoses eg atopic eczema
and psoriasis, an increased number of factor XIIIa positive cells in the upper
dermis, closely associated with lymphocytes, has been described.
Refer to page 28 for the Bond ready-to-use format.
Fas-Associated Death Domain Protein
Clone 64A6
1 mL lyophilized NCL-FADD F P (HIER)
Fas-Associated Death Domain Protein (FADD), also known as Mort-1, is a
cytoplasmic death domain-containing protein (23 kD) which interacts with
the intracellular death domain of Fas and initiates apoptosis. The interaction
of FADD and Fas through their C-terminal death domains unmasks the Nterminal
effector domain of FADD allowing it to recruit caspase-8 to the Fas
signalling complex and thereby activating a cysteine protease cascade. The
end result of this pathway is a cell death mechanism characterized by
nuclear and cytoplasmic condensation and DNA fragmentation. FADD exists
in the cytoplasm of normal cells but does not induce cell death unless
present in high concentration. It has been reported to be expressed in
mammalian testis where it is implicated in the modulation of testicular
homeostasis by controlling germ cell apoptosis. FADD is also reported to be
downregulated in hepatocellular carcinomas, tumors known to be resistant
to Fas-mediated apoptosis.
Human fetus: immunohistochemical staining for Fas-associated death domain protein using
NCL-FADD. Note intense membrane and cytoplasmic staining of developing fetal skin, internal
components and organs. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies