Labelling Review row-Online

Chromogranin A
Clone 5H7
1 mL, 0.1 mL lyophilized NCL-CHROM-430 P (HIER)
7 mL Bond ready-to-use PA0430 P (HIER)
Chromogranin A is a 68 kD acidic protein which is reported to be widely
expressed in neural tissues and in secretory granules of human endocrine
cells eg parathyroid gland, adrenal medulla, anterior pituitary gland, islet
cells of the pancreas and C cells of the thyroid. Chromogranin A expression
has been reported in neuroendocrine tumors such as pituitary adenomas,
islet cell tumors, phaeochromocytomas, medullary thyroid carcinomas,
Merkel cell tumors and carcinoids.
Refer to page 25 for the Bond ready-to-use format.
Human insulinoma: immunohistochemical staining for chromogranin A using NCL-CHROM-430.
Note intense cytoplasmic staining of neoplastic islet cells. Paraffin section.
c-jun Oncoprotein
Clone DK4
1 mL lyophilized NCL-cJUN F P (Enzyme)
c-jun is the normal cellular homolog of the transforming gene of avian
sarcoma virus 17 and is a member of the early-response gene family. The cjun
oncogene encodes a nuclear protein, p39, which is a major component
of the transcription factor AP1 and interacts with the c-fos oncogene
product forming a transacting heterodimer. c-jun oncoprotein plays an
important role in the regulation of gene expression and signal transduction
processes. Alterations in c-jun expression may affect the transcriptional
initiation of specific target genes and as a consequence may affect normal
cell growth and function.
Human breast carcinoma: immunohistochemical staining for c-jun oncoprotein using
NCL-cJUN. Note nuclear staining of tumor cells. Paraffin section.
c-kit Oncoprotein (CD117)
Clone T595
1 mL, 0.1 mL lyophilized NCL-CD117 P (HIER)
1 mL liquid NCL-L-CD117 P (HIER)
7 mL ready-to-use RTU-CD117 P (HIER)
Clone 57A5D8
1 mL, 0.1 mL lyophilized NCL-cKIT F
Reference Range
The c-kit proto-oncogene encodes a transmembrane receptor with tyrosine
kinase activity, c-kit (CD117), which is closely-related to the platelet-derived
growth factor receptor family. c-kit plays a role during hematopoiesis,
gametogenesis and melanogenesis. The expression of CD117 antigen is of
particular interest in the study of gastrointestinal stromal tumors (GIST),
small lung cell carcinomas and in melanomas.
Human skin: immunohistochemical staining for c-kit oncoprotein (CD117) using NCL-CD117.
Note membrane staining of a proportion of melanocytes and mast cells. Paraffin section.
Clusterin (Apolipoprotein J)
Clone 7D1
1 mL lyophilized NCL-CLUSTERIN P (HIER)
Clusterin is also known as apolipoprotein J, complement lysis inhibitor, gp80,
glycoprotein III, SGP-2, SP 40, TRPM2 and T64. It is a ubiquitous, multifunctional
protein of 80 kD comprising of two disulfide-linked subunits, alpha
and beta. It is implicated in numerous biological processes including sperm
maturation, lipid transport, regulation of the complement cascade,
membrane recycling, cell death, immune regulation, cell adhesion and
morphological transformation. In pathological conditions, it is an amyloid
associated protein co-localizing with fibrillar deposits in systemic and
localized amyloid disorders. In Alzheimer's disease, clusterin is reported to
be present in amyloid plaques and cerebrovascular deposits. In breast
cancers, clusterin expression is reported to be correlated with tumor size
and, when upregulated, correlated inversely with apoptotic index. This
suggested that clusterin expression was not a prerequisite to cellular death
by apoptosis. Clusterin has also been reported to be expressed in anaplastic
large cell lymphoma.
Human tonsil: immunohistochemical staining for clusterin (apolipoprotein J) using
NCL-CLUSTERIN. Note intense membrane staining of follicular cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies