Labelling Review row-Online

CD40
Clone 11E9
1 mL, 0.1 mL lyophilized NCL-CD40 P (HIER) W
The CD40 antigen is a single chain glycoprotein with a calculated molecular
weight of 27 kD. It is known to be a member of the tumor necrosis factor/
nerve growth factor superfamily and shows a significant homology to the
Hodgkin's disease-associated antigen, CD30. The precise function of the
CD40 antigen is unknown but it appears to be involved in the transduction of
regulatory signals for cellular functions such as B cell proliferation and
differentiation. It is also important in the prevention of apoptosis of germinal
center B cells. The CD40 antigen is reported to be found on mature B cells
(except plasma cells), most B cell leukemias and lymphomas, interdigitating
reticulum cells, follicular dendritic cells and Reed Sternberg cells. Outside
the immune system, CD40 antigen is reported to be expressed on some
epithelial cells of certain carcinomas and in malignant melanomas.
CD41 (GPIIb/IIIa)
Clone M148
1 mL lyophilized NCL-CD41 F
The CD41 antigen, also known as GPIIb/IIIa, is reported to be expressed
early in megakaryocyte maturation and in megakaryoblastic leukemias and
is absent or defective in platelets from patients with Glanzmann's thrombasthenia.
The CD41 antigen is involved in fibrinogen binding, clot retraction
and platelet aggregation.
Human tonsil: immunohistochemical staining for CD41 antigen (GPIIb/IIIa) using NCL-CD41.
Note staining of aggregated platelets within the blood vessel. Frozen section.
CD42b (GPIb)
Clone MM2/174
1 mL lyophilized NCL-CD42b F P (HIER)
The CD42b glycoprotein, also known as GPIb, is a co-factor of ristocetininduced
aggregation and is involved in the binding of platelets to blood
vessel walls. The CD42b antigen is reported to be expressed on platelets and
on megakaryocytes in bone marrow and in megakaryoblastic leukemias. The
absence of CD42b antigen on platelets is reported to be a possible indicator
of Bernard-Soulier disease.
CD43
Clone MT1
1 mL lyophilized NCL-MT1 F P (Enzyme) W
1 mL liquid NCL-L-MT1 F P (Enzyme) W
7 mL ready-to-use RTU-MT1 F P (Enzyme)
7 mL Bond ready-to-use PA0938 P (HIER)
Clone MT1 produces superior staining on paraffin sections.
The CD43 antigen is expressed on the membrane and in the cytoplasm of T
cells and cells of myeloid lineage. The molecule itself exhibits molecular
weight heterogeneity with bands of 90 to 140 kD observed on SDS-PAGE
between different cell lines. Cells expressing the CD43 antigen are reported
to include normal and neoplastic T cells. A small proportion of B cell chronic
leukemias and centrocytic lymphomas are also reported to express CD43
antigen.
Product Specific Information
Enzyme pretreatment may enhance staining with clone MT1 in some cases.
Refer to page 22 for the Bond ready-to-use format.
Human mantle cell lymphoma: immunohistochemical staining for CD43 antigen using NCL-MT1.
Note intense membrane staining of tumor cells. Paraffin section.
CD44 (H-CAM)
Clone DF1485
1 mL, 0.1 mL lyophilized NCL-CD44-2 F P (HIER) C
The CD44 antigen (H-CAM) is an 80 to 95 kD transmembrane glycoprotein
with extensive O-linked glycosylation. The antigen is a cell surface receptor
for hyaluronate, suggesting a role in the regulation of cell substrate
interactions as well as cell migration. CD44 antigen is reported to be
expressed on T cells, B cells, monocytes, granulocytes, erythrocytes and
weakly on platelets. Other CD44 antigen positive cell types are reported to
include epithelial cells, glial cells, fibroblasts and myocytes. Increased
expression of CD44 antigen is found on some carcinomas and it has been
reported that transition of tumor cell lines from non-metastatic to metastatic
may be associated with changes in the expression of CD44 antigen variants.
Human squamous cell carcinoma of breast: immunohistochemical staining for CD44 antigen
(H-CAM) using NCL-CD44-2. Note intense membrane staining of tumor cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies