Labelling Review row-Online

Platelet-Derived Endothelial Growth
Factor
Clone P-GF.44C
1 mL, 0.1 mL lyophilized NCL-PDEGF P (HIER) W
Angiogenesis, the formation of new blood vessels from an existing vascular
bed is a complex multi-step process. It is controlled by a number of
angiogenic factors, one of which is platelet-derived endothelial growth
factor (PDEGF) also shown to be thymidine phosphorylase (TP).
Angiogenesis is tightly regulated and is observed only transiently during
reproduction, development and wound healing. PDEGF is reported to be
expressed in the nucleus and cytoplasm, the highest expression being
described in macrophages, stromal cells, glial cells and some epithelia. No
expression is reported in gastro-intestinal epithelium, smooth muscle,
adrenal glands, lung and testis. The high expression in macrophages and
skin may be important for total body thymidine homeostasis.
Western blot: detection of platelet-derived endothelial growth factor (53 kD) using NCL-PDEGF.
Lane A, molecular weight markers. Lane B, human tonsil immunoblotted with NCL-PDEGF.
Pneumolysin
Clone 9.1/2/3/6
1 mL lyophilized NCL-SPNm W
Pneumolysin is a 53 kD species-specific protein produced by all important
strains of Streptococcus pneumoniae (pneumococcus), an organism which
causes a wide range of human disease, from sinusitis and pneumonia to
septicaemia and meningitis.
Western blot: detection of pneumolysin (53 kD) using NCL-SPNm. Lane A, molecular weight
markers. Lanes B to E, Streptococcus pneumoniae culture-positive sputum samples. Lane F,
Streptococcus pneumoniae culture-negative but pneumolysin PCR-positive nasopharyngeal
secretion. Lane G, Streptococcus pneumoniae culture-positive fluid from an infected joint.
Polyomavirus
Clone 3.1.1
1 mL, 0.1 mL lyophilized Polyomavirus (JC/BK viruses)
NCL-JCBK IO
JC virus, a Polyomavirus of the Papovaviridae was first isolated from the
brain of an individual with a rare demyelinating condition. JC and BK viruses
are oncogenic in newborn hamsters and transform mammalian cells in vitro
but there is no evidence that they cause human cancer. JC virus is
ubiquitous in humans and may be occasionally shed in the urine without any
apparent symptoms during pregnancy or immunosuppression. Unlike BK
virus, also a Polyomavirus, JC virus may cause a lethal disease, progressive
multifocal leukoencephalopathy (PML). PML is a rare subacute
demyelinating disease of the CNS which is seen mainly as a complication of
advanced disseminated malignant conditions such as Hodgkin's disease or
chronic lymphocytic leukemia and also in HIV infection or following
immunosuppression for organ transplantation. The target cell of JC virus is
the oligodendrocyte in which the virus undergoes a lytic productive
infection. JC virus may be cultured from infected urine or brain in human
fetal glial cells or detected directly in biopsy or post-mortem tissue.
Product Specific Information
NCL-JCBK, specific for both JC virus and BK virus, is effective in
immunocytochemistry. NCL-JCBK does not cross-react with Respiratory
syncytial virus, Parainfluenza virus types 1, 2, 3 and 4b, Adenovirus, Varicellazoster
virus, Herpes simplex virus types 1 and 2, Mumps virus, Measles virus,
echovirus 19, Coxsackie B4 virus, Poliovirus types 1, 2 and 3 and Influenza
virus types A and B.
Prealbumin
Polyclonal
1 mL lyophilized NCL-PREp F P (Enzyme)
Prealbumin, also known as transthyretin, is a 55 kD molecule synthesized in
the liver. Prealbumin serves as a transport protein for thyroid hormones and
vitamin A. Variant prealbumin has been identified as the major fibril subunit
protein in several hereditary forms of systemic amyloidosis, including
familial amyloid polyneuropathy types I and II.
Human small cell carcinoid tumor: immunohistochemical staining for prealbumin using
NCL-PREp. Note cytoplasmic staining of tumor cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies