Labelling Review row-Online
Labelling Review row-Online
Labelling Review row-Online
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Corynebacterium diphtheriae<br />
100 Tests primer set NCL-CD-PS O<br />
Large outbreaks of diphtheria in eastern Europe and modern travel have led<br />
to an increased awareness of Corynebacterium diphtheriae. Most western<br />
isolates are still non-toxigenic, but toxin tests require experience and<br />
expertise.<br />
Product Specific Information<br />
The Corynebacterium diphtheriae primer set, NCL-CD-PS, consists of a pair<br />
of oligonucleotide primers (CD-1 and CD-2) which amplify a 246bp region of<br />
the toxin gene alpha subunit. Also provided is an internal control DNA for<br />
aiding in the detection of false-negative PCR results. The internal control DNA<br />
may be added to the test PCR, where it will amplify with the same primers but<br />
yield a product of different size.<br />
Agarose gel: electrophoresis of PCR products using NCL-CD-PS. Lane A, 100bp ladder<br />
(Promega). Lane B, Reagent blank. Lanes C-F and H, five toxigenic Corynebacterium<br />
diphtheriae isolates. Lane G, non-toxigenic Corynebacterium diphtheriae isolate. The<br />
polymerase chain reaction (PCR) process is covered by patents owned by Hoffman Laroche<br />
Inc. and Hoffman La-Roche Ltd. Purchase of these products does not carry a licence to<br />
perform the PCR process.<br />
Mycobacterium tuberculosis<br />
100 Tests primer set NCL-MT-PS O<br />
RUO RUO<br />
There is a rising incidence of tuberculosis, an emergence of drug-resistant<br />
strains of Mycobacterium tuberculosis and an overlap with AIDS. Direct<br />
detection and identification of Mycobacterium tuberculosis in sputum is<br />
possible by PCR. Some low level infections may be missed (but later<br />
detected by culture), but it is reported that PCR will certainly identify all<br />
strains of Mycobacterium tuberculosis detected by conventional<br />
microscopy (60 to 80 percent).<br />
Product Specific Information<br />
The Mycobacterium tuberculosis primer set, NCL-MT-PS, consists of a pair<br />
of oligonucleotide primers (MT-1 and MT-2) which amplify the 123bp insertion<br />
sequence IS6110. The sequence is unique to members of the Mycobacterium<br />
tuberculosis complex (MTBC) and is present as a sequence in multiple copies<br />
in many common isolates. Also provided is an internal control DNA for aiding<br />
in the detection of false-negative PCR results.<br />
Agarose gel: electrophoresis of PCR products using NCL-MT-PS. Lane A, 123bp ladder (Sigma).<br />
Lane B, internal control only. Lanes C, D and E, Mycobacterium tuberculosis, Mycobacterium<br />
avium and Mycobacterium intracellulare isolates, respectively. Lanes F and G, Mycobacterium<br />
tuberculosis-positive human sputa. Lane H, Mycobacterium tuberculosis-positive, formalinfixed,<br />
paraffin-embedded human lung. The polymerase chain reaction (PCR) process is<br />
covered by patents owned by Hoffman Laroche Inc. and Hoffman La-Roche Ltd. Purchase<br />
of these products does not carry a licence to perform the PCR process.<br />
F Frozen I Immunofluorescence E Electron microscopy<br />
P Paraffin C Flow cytometry O Other applications<br />
W Western blotting<br />
/ 189<br />
Primer Sets