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Primary Antibodies
Epstein-Barr virus-Induced Gene 3
Protein
Clone EL8
1 mL lyophilized NCL-EBI-3 F P (HIER)
Epstein-Barr virus (EBV)-associated Hodgkin's lymphoma (HL) and nasopharyngeal
carcinoma (NPC) usually occurs in individuals without clinically
apparent deficiencies in anti-viral immunity. Despite expressing viral
proteins, both tumors are apparently able to escape EBV-specific immunity
in vivo. EBI-3 is an EBV-induced cytokine homologous to the interleukin 12
p40 subunit which can heterodimerize with the interleukin 12 p35 subunit.
Researchers have suggested that EBI-3 protein may function to antagonize
interleukin 12 and to inhibit the development of a Th1 immune response. It
has been reported that EBI-3 protein is strongly expressed in Hodgkin's
Reed Sternberg (RS) cells in approximately 96 percent of HL cases,
independently of the EBV status of the tumor cells. EBI-3 protein has also
been reported to be detected in a small percentage of epithelial tumor cells
of NPC biopsies but not in Burkitt's lymphomas. EBI-3 protein may be an
additional component of the repertoire employed by Hodgkin's RS cells to
inhibit and effect anti-tumor or anti-viral immune response. EBI-3 protein
expression has also been reported in spleen, tonsil, mature dendritic cells,
colonic mucosa and at high levels in full term placenta.
Hodgkin's lymphoma: immunohistochemical staining for Epstein-Barr virus-induced gene 3
protein using NCL-EBI-3. Note cytoplasmic staining of infected cells. Paraffin section.
Epstein-Barr virus (LMP-1)
Clone CS1/CS2/CS3/CS4 cocktail
1 mL, 0.1 mL lyophilized NCL-EBV-CS1-4 F P (Enzyme)
Epstein-Barr virus (EBV) is one of the eight known human herpes viruses
and belongs to the Gammaherpes viriniae, the same subfamily as human
herpesvirus type 8 (HHV-8). Herpes viruses have large double strand DNA
genomes and are complex viruses often encoding over 35 proteins including
enzymes involved in nucleic acid metabolism, DNA synthesis and protein
processing in addition to viral structural proteins. These viruses are capable
of entering a latent phase where the host shows no visible signs of infection
and levels of infectious agent become very low. During latency, viral gene
expression is restricted to only a few genes. Latent membrane protein (LMP-
1) is a 60 kD protein encoded by the BNLF1 gene of EBV.
Product Specific Information
NCL-EBV-CS1-4 is a cocktail of four monoclonal antibodies; clones CS1, CS2,
CS3 and CS4.
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Epstein-Barr virus (nuclear antigen 2)
Clone PE2
1 mL, 0.1 mL lyophilized NCL-EBV-PE2 FW
Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) is an EBV-encoded
nuclear protein of 82 kD. EBNA2 is essential for growth transformation of
B lymphocytes and has been shown to modulate the activity of several viral
and cellular promoters.
Estrogen Receptor
Clone 6F11
2 mL lyophilized NCL-ER-6F11/2 F P (HIER) W C
2 mL liquid NCL-L-ER-6F11/2 F P (HIER) W C
1 mL. 0.1mL lyophilized NCL-ER-6F11 F P (HIER) W C
1 mL liquid NCL-L-ER-6F11 F P (HIER) W C
7 mL ready-to-use RTU-ER-6F11 F P (HIER) W
7 mL Bond ready-to-use PA0151 P (HIER)
Estrogen receptor (ER) content of breast cancer tissue is an important
parameter in the prediction of prognosis and response to endocrine therapy.
The introduction of highly specific monoclonal antibodies to ER has allowed
the determination of receptor status of breast tumors to be carried out in
routine histopathology laboratories.
Product Specific Information
Clone 6F11 is raised to the full length alpha form of the estrogen receptor
molecule present on human ER antigen, located in the nucleus of ER positive
normal and neoplastic cells. Clone 6F11 has been extensively tested (Bevitt D
J et al. Journal of Pathology. 183 : 228-232 (1997)). Further publications exist
that discuss the sensitivity of clone 6F11 (Kauffman O et al. Modern Pathology
11(4):357-363 (1998)) and Kaplan P A et al. American Journal of Clinical
Pathology 123: 276-280 (2005). NCL-ER-6F11/2 and NCL-L-ER-6F11/2 are more
economic options for high volume users of clone 6F11.
Refer to page 28 for the Bond ready-to-use format.
Human breast carcinoma: immunohistochemical staining for estrogen receptor using
NCL-L-ER-6F11. Note intense nuclear staining of tumor cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range