Labelling Review row-Online

Lambda Light Chain
Clone SHL53 New!
1 mL, 0.1 mL liquid NCL-L-LAM-578 P (HIER)
Clone HP-6054
1 mL, 0.1mL lyophilized NCL-LAM FPW
Polyclonal
1 mL lyophilized NCL-LAMp P (Enzyme) W
The basic structure of an immunoglobulin molecule consists of two identical
heavy chains, either �, �, �, � or �, and two identical light chains, either
kappa or lambda. Any heavy chain can associate with either light chain but
on any immunoglobulin molecule both light chains are of the same type. The
ratio of kappa and lambda light chains varies between Ig classes and
subclasses. In a polyclonal population the ratio of kappa to lambda bearing
B cells is approximately 2:1, with individual B cells thought to express kappa
or lambda light chains, never both. The majority of kappa and lambda chains
are bound to heavy chain immunoglobulin, however in normal individuals
low levels of free light chain are present in serum. The occurrence of a
mixture of kappa and lambda chain expressing cells suggests a polyclonal
population and a reactive or nonneoplastic proliferation of B cells.
Tonsil: immunohistochemical staining with Lambda Light Chain using NCL-L-LAM-578. Note
intense cytoplasmic staining of plasma cells. Paraffin section.
Lamin
Reference Range
Clone 636
1 mL, 0.1 mL lyophilized lamin A/C NCL-LAM-A/C F P (HIER)
W
The nuclear lamina is a karyoskeletal structure composed of intermediate
filament type proteins called lamins. It underlies the inner nuclear
membrane and confers mechanical stability to the nuclear envelope. The
human lamina consists of four major types of lamin, namely A, B1, B2 and C.
The loss of lamin A expression has been reported to occur in small cell lung
cancers.
Product Specific Information
NCL-LAM-A/C reacts with lamins A and C in human, cow and pig tissues.
Laminin
Clone LAM-89
0.5 mL lyophilized NCL-LAMININ F P (Enzyme)
Laminin is a large (850 kD) disulfide-bonded heterotrimer, cross-shaped,
glycoprotein which is organized within the meshwork of basement
membranes such as those associated with epithelia, surrounding blood
vessels, nerves and underlying pial sheaths of the brain. It is reported to be
expressed in the extracellular matrix in sites other than basement
membranes during early stages of development and is localized to specific
types of neurons in the central nervous system during both embryonic and
adult development. Laminin interacts with receptors on cell surfaces, an
interaction which results in changes in the behavior of cells such as
attachment to a substrate, migration and neurite outgrowth during
embryonic development and regeneration.
Human placenta: immunohistochemical staining for laminin using NCL-LAMININ. Note staining
of basement membranes of blood vessels. Paraffin section.
Langerin
Clone 12D6
1 mL, 0.1 mL lyophilized NCL-LANGERIN P (HIER)
Langerin is a type II transmembrane C-type lectin which has mannose-binding
specificity. It is a 40 kD protein restricted to Langerhans cells that is involved in
the internalization of cell surface material in these immature dendritic cells.
Dendritic cells are antigen-presenting cells that are required for initiation of a
specific T cell-driven immune response. These cells are found in nonlymphoid
tissue as immature cells whose primary function is to capture antigen through
specialized surface membrane endocytic struc-tures or through
macropinocytosis. The dendritic cells migrate to secondary lymphoid tissue and
mature into efficient antigen presenting cells. A part of the maturation process
includes the loss of adhesion receptors such as E-cadherin and the
disappearance of Birbeck granules. Although Langerin is reported to be located
on the cell surface, it can be rapidly internalized following ligand capture into
Birbeck granules. In fact, Langerin is a potent inducer of membrane
superimposition and zippering leading to Birbeck granule formation. In reports it
has been suggested that the induction of Birbeck granules is a consequence of
the antigen-capture function of Langerin allowing passage into these organelles
and providing access to a non-classical antigen processing pathway.
Human basal cell carcinoma: immunohistochemical staining for langerin using
NCL-LANGERIN. Note membrane and cytoplasmic staining of Langerhans cells within the
tumor. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies