Labelling Review row-Online

Product Specific Information
Using retrieval solutions other than that recommended for Clone JCM182 in
the datasheet may increase background reactivity.
Refer to page 21 for the Bond ready-to-use format.
Hodgkin's lymphoma: immunohistochemical staining for CD30 antigen using NCL-L-CD30-591.
Note membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg
cells. Paraffin section.
CD31 (PECAM-1)
Clone 1A10
1 mL, 0.1 mL lyophilized NCL-CD31-1A10 P (HIER)
7 mL Bond ready-to-use PA0250 P (HIER)
CD31 antigen (PECAM-1) is a single chain transmembrane glycoprotein with
a molecular weight of 130 to 140 kD. The CD31 molecule is expressed on the
surface of platelets, monocytes, granulocytes, B cells and at the endothelial
intracellular junction. The molecule has an extracellular domain that
contains six Ig-like homology units of C2 subclass, typical of cell to cell
adhesion molecules. This domain mediates endothelial cell to cell adhesion,
plays a role in endothelial contact and may serve to stabilize the endothelial
cell monolayer. The CD31 molecule also has a cytoplasmic domain with
potential sites for phosphorylation after cellular activation. The properties of
CD31 antigen suggest that it is involved in interactive events during
angiogenesis, thrombosis and wound healing. Angiogenesis is essential for
tumor growth and metastases.
Refer to page 21 for the Bond ready-to-use format.
Human glomangioma: immunohistochemical staining for CD31 antigen (PECAM-1) using
NCL-CD31-1A10. Note intense membrane staining of endothelial cells. Paraffin section.
CD33
Clone PWS44
1 mL, 0.1 mL liquid NCL-L-CD33 P (HIER) W
7 mL Bond ready-to-use PA0555 P (HIER)
CD33 antigen is reported to appear on myelomonocytic precursor cells after
CD34 antigen expression. It then continues to be expressed on both the
myeloid and monocyte lineages, although it is reported to be absent on
granulocytes. It has been reported that expression of CD33 is restricted to
monocytes, premyelocytes, myeloid blasts, some acute undifferentiated
leukemias and acute lymphoblastic leukemias. The expression of CD33
antigen has been demonstrated to be an important marker for distinguishing
myeloid from the lymphoid leukemias.
Refer to page 22 for the Bond ready-to-use format.
Acute myeloid leukemia: immunohistochemical staining for CD33 antigen using NCL-L-CD33.
Note intense cytoplasmic and membrane staining of malignant cells. Paraffin section.
CD34 (Endothelial Cell Marker)
Reference Range
Clone QBEnd/10
1 mL, 0.1 mL lyophilized NCL-END F P (Enzyme) C
1 mL liquid NCL-L-END F P (Enzyme) C
7 mL ready-to-use RTU-END F P (Enzyme)
7 mL Bond ready-to-use PA0212 P (HIER)
Reference Range
CD34 antigen is a single chain transmembrane glycoprotein with a
molecular weight of 110 kD. The CD34 protein is selectively expressed on
human lymphoid and myeloid haemapoietic progenitor cells. The CD34
protein is also expressed on vascular endothelium.
Product Specific Information
Enzyme digestion of paraffin sections is recommended with Clone QBEnd/10
in preference to heat induced epitope retrieval as it produces stronger
staining and reduces background elastin staining.
Refer to page 22 for the Bond ready-to-use format.
Human tonsil: immunohistochemical staining for CD34 antigen using NCL-END. Note intense
staining of neoplastic endothelial cells and absence of staining of stromal cells. Paraffin
section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies