Labelling Review row-Online

CD45RB
Clone MEM55
1 mL lyophilized NCL-CD45RB F P (HIER)
The CD45 molecule is reported to be found on all cells of hematopoietic
origin, except erythrocytes. The various isoforms are expressed differently
on various lymphoid cell types and are termed CD45RA, CD45RB, CD45RC
and CD45RO. Low expression of CD45RB on CD45RO positive T lymphocytes
defines a subset of highly differentiated T lymphocytes which accumulate in
vivo within affected rheumatoid arthritic joints. The percentage of these cell
types is also reported to be increased in the circulation of individuals with
acute EBV infection and it is thought that these cells have a migratory
advantage and are selectively recruited to sites of inflammation. CD45RB
antigen is also reported to be found on B cells, monocytes, macrophages
and is expressed weakly on granulocytes.
Product Specific Information
The heat induced epitope retrieval (HIER) technique may enhance staining in
some cases.
Human stomach, B cell lymphoma: immunohistochemical staining for CD45RB antigen using
NCL-CD45RB. Note intense membrane staining of neoplastic lymphoid cells. Paraffin section.
CD45RO
Clone UCHL1
1 mL, 0.1 mL lyophilized NCL-UCHL1 F P (HIER) C
1 mL liquid NCL-L-UCHL1 F P (HIER) C
7 mL ready-to-use RTU-UCHL1 F P (HIER)
7 mL Bond ready-to-use PA0146 P (HIER)
The CD45RO molecule, a 180 kD isoform of CD45, is reported to be expressed
on 48 percent of peripheral blood T lymphocytes, 37 percent of CD4 positive
lymphocytes, 80 percent of thymocytes and on the majority of T cell
malignancies. Monocytes and granulocytes show surface expression of the
antigen whereas tissue macrophages exhibit cytoplasmic expression. The
heat induced epitope retrieval (HIER) technique may enhance staining in
some cases.
Refer to page 23 for the Bond ready-to-use format.
CD54 (ICAM-1)
Clone 23G12
1 mL, 0.1 mL lyophilized NCL-CD54-307 P (HIER)
See also ICAM-1 (CD54) on page 123.
CD56 (NCAM)
Clone CD564
1 mL, 0.1 mL lyophilized NCL-CD56-504 P (HIER)
7 mL Bond ready-to-use PA0191 P (HIER)
Clone 1B6
1 mL, 0.1 mL lyophilized NCL-CD56-1B6 P (HIER) W
1 mL liquid NCL-L-CD56-1B6 P (HIER) W
1 mL ready-to-use RTU-CD56-1B6 P (HIER)
Clone CD564 was developed to produce superior staining on paraffin
sections.
The neural cell adhesion molecules are a family of closely-related cell
surface glycoproteins thought to play a role in embryogenesis, development
and contact-mediated interactions between neural cells. The CD56 antigen
(NCAM) consists of four major isoforms generated by differential splicing of
the RNA transcript from a single gene located on chromosome 5. The CD56
antigen is expressed on neurons, astrocytes, Schwann cells, NK cells and a
subset of activated T lymphocytes.
Refer to page 23 for the Bond ready-to-use format.
Human small intestine: immunohistochemical staining for CD56 antigen (NCAM) using
NCL-CD56-564. Note membrane staining of enteric ganglion cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies