Labelling Review row-Online

Primary Antibodies
CD14
Clone 7
1 mL, 0.1 mL lyophilized NCL-CD14-223 P (HIER) W
1 mL liquid NCL-L-CD14-223 P (HIER) W
CD14 antigen is a glycosyl-phosphatidylinositol (GPI)-linked glycoprotein
with a molecular weight of 55 kD. The CD14 antigen is reported to be
expressed on cells of the myelomonocytic lineage including monocytes,
macrophages and Langerhans cells. Low expression is also reported on
neutrophils and on B cells. CD14 antigen is a receptor for bacterial
lipopolysaccharide (LPS, endotoxin) and the lipopolysaccharide binding
protein (LBP). LBP and CD14 antigen serve two physiological roles. These
proteins act as opsonin and opsonic receptor, respectively, to promote the
phagocytic uptake of bacteria or LPS-coated particles by macrophages.
Human uterus: immunohistochemical staining for CD14 antigen using NCL-CD14-223. Note
membrane staining of macrophages within an endometrial gland and lymphocytes in the
stroma. Paraffin section.
CD15
Clone BY87
1 mL, 0.1 mL lyophilized NCL-CD15 F P (HIER/Enzyme)
1 mL liquid NCL-L-CD15 F P (HIER/Enzyme)
7 mL ready-to-use RTU-CD15 F P (HIER/Enzyme)
Clone Carb-1
7 mL Bond ready-to-use PA0039 P (HIER)
CD15 antigen, also known as X-hapten, is reported to be expressed on 90
percent of circulating human granulocytes, 30 to 60 percent of circulating
monocytes and is absent from normal lymphocytes. The CD15 antigen is also
expressed on Reed Sternberg cells of Hodgkin's disease and some
leukemias.
Product Specific Information
Please note that either enzyme pretreatment or heat induced epitope
retrieval (HIER) temperature antigen unmasking using 1mM EDTA (pH8.0)
solution may be used on fixed, paraffin-embedded tissue. The choice of
antigen unmasking technique to provide the best result should be determined
by the user.
Refer to page 19 for the Bond ready-to-use format.
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Hodgkin's disease: immunohistochemical staining for CD15 antigen using NCL-CD15. Note
membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg cells.
Paraffin section.
CD16
Clone 2H7
1 mL, 0.1 mL lyophilized NCL-CD16 P (HIER)
CD16 antigen has a molecular weight of 50 to 70 kD and is a low affinity Fc
receptor for complexed IgG, Fc/gamma RIII, expressed on natural killer (NK)
cells, granulocytes, activated macrophages and a subset of T cells expressing
alpha-beta or gamma-delta T cell antigen receptors. The CD16 antigen
exists both as a glycosyl-phosphatidylinositol (GPI)-anchored protein in
polymorphonuclear cells and as a transmembrane protein in NK cells.
Human colon, ulcerative colitis: immunohistochemical staining for CD16 antigen using
NCL-CD16. Note intense membrane staining of infiltrating natural killer cells, granulocytes
and activated macrophages. Paraffin section.
CD19
Clone BT51E
1 mL, 0.1 mL liquid NCL-L-CD19-163 P (HIER) W
7 mL Bond ready-to-use PA0843 P (HIER)
Clone 4G7/2E
1 mL, 0.1 mL lyophilized NCL-CD19-2 FC
Clone BT51E was developed to be effective on formalin-fixed, paraffinembedded
tissue sections.
CD19 is a member of the immunoglobulin superfamily and has two Ig like
domains. It is a single chain glycoprotein present on the surface of B
lymphocytes and follicular dendritic cells of the hematopoietic system. CD19
is a crucial regulator in B cell development, activation and differentiation.
On B cells, CD19 associates with CD21, CD81 and CD225 (Leu-13) forming a
signal transduction complex. CD19 is expressed from the earliest
recognizable B cell lineage stage, through development to B cell
differentiation but is lost on maturation to plasma cells.
Refer to page 20 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range