Labelling Review row-Online

Primary Antibodies
p53 Protein (CM5)
Polyclonal
0.2 mL lyophilized NCL-p53-CM5p P (HIER) W
The accumulation of p53 protein in response to genotoxic stress in vitro is
well established and appears to induce growth arrest and apoptosis by the
transcriptional regulation of other genes and possibly by other direct
mechanisms.
Product Specific Information
NCL-p53-CM5p is specific for mouse and rat p53 protein.
Western blot: detection of p53 protein (53 kD) using NCL-p53-CM5p. Lane A, molecular weight
markers. Lane B, T3T3 mouse cell line immunoblotted with NCL-p53-CM5p.
p53 Protein (DO-1)
Clone DO-1
1 mL lyophilized NCL-p53-DO1 F P (HIER) W
In man, the p53 gene is located on the small arm of chromosome 17.
Alterations of the p53 tumor suppressor gene are a common feature of
human malignancies. A normal function of this gene is to induce apoptosis
after DNA damage and, therefore, its activation can permit the survival of
cells that have sustained genetic damage.
Product Specific Information
Clone DO-1 recognizes both wild type and mutant forms of human p53 protein
under denaturing and non-denaturing conditions. The epitope to which NCLp53-DO1
maps is sited at the N-terminus at amino acids 20 – 25.
Human colonic adenocarcinoma: immunohistochemical staining for p53 protein using
NCL-p53-DO1. Note intense nuclear staining of tumor cells. Paraffin section.
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p53 Protein (DO-7)
Clone DO-7
1 mL, 0.1 mL lyophilized NCL-p53-DO7 FPWC
1 mL liquid NCL-L-p53-DO7 FPWC
7 mL ready-to-use RTU-p53-DO7 FP
7 mL Bond ready-to-use PA0057 P (HIER)
Product Specific Information
This monoclonal antibody recognizes both wild type and mutant forms of
human p53 protein under denaturing and non-denaturing conditions. The
epitope recognized by clone DO-7 can be destroyed by prolonged fixation in
buffered formalin. The heat induced epitope retrieval technique may improve
staining in some cases.
Refer to page 38 for the Bond ready-to-use format.
Human skin, basal cell carcinoma: immunohistochemical staining for p53 protein using the
ready-to-use reagent RTU-p53-DO7. Note intense nuclear staining of tumor cells. Paraffin
section.
p53 Protein (Phosphospecific)
Clone FP3-2
1 mL lyophilized NCL-p53-PHOS P (HIER) W
p53 protein plays a vital role in suppressing the development of cancer.
Post-translational modification by phosphorylation has been proposed to be
an important regulatory mechanism of p53 function. Several p53
phosphorylation sites fall within or closely flank a number of functional
domains within the p53 molecule including transcriptional transactivating,
DNA binding and oligomerization sequences. Several p53 protein mutants
are less phosphorylated than wild type p53 protein when expressed in vivo
which may suggest that phosphorylation positively regulates DNA binding.
However, other experiments have also suggested that phosphorylation may
also negatively regulate DNA binding.
Product Specific Information
NCL-p53-PHOS is specific for the phosphorylated site on human p53 protein
at Ser392 within the non-specific DNA binding/oligomerization region. The
functional significance of phosphorylation at this site only, remains unclear.
Human colonic adenocarcinoma: immunohistochemical staining for p53 protein using
NCL-p53-PHOS. Note intense nuclear staining of tumor cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range