Labelling Review row-Online

Primary Antibodies
CD7
Clone LP15
1 mL, 0.1 mL liquid NCL-L-CD7-580 P (HIER)
7 mL Bond ready-to-use PA0266 P (HIER)
The CD7 molecule is a membrane-bound glycoprotein of 40 kD and is the
earliest T cell specific antigen to be expressed in lymphocytes. CD7 antigen
is also the only early marker to persist throughout differentiation. The
function and role of the CD7 molecule has not yet been fully identified,
although the activation of T cells with gamma/delta receptors has been
proposed based on mAb-induced activation. CD7 antigen is reported to be
found on the majority of peripheral blood T cells, most natural killer cells and
thymocytes.
Product Specific Information
Clone LP15 was developed to provide superior staining to clone CD7-272 on
paraffin sections.
Refer to page 18 for the Bond ready-to-use format.
Human thymus: immunohistochemical staining for CD7 antigen using NCL-L-CD7-580.
Note intense staining of cortical thymocytes. Paraffin section.
CD8
Clone 1A5
1 mL, 0.1 mL lyophilized NCL-CD8-295 F P (HIER) W
1 mL liquid NCL-L-CD8-295 F P (HIER) W
7 mL ready-to-use RTU-CD8-295 F P (HIER)
Clone 4B11
1 mL, 0.1 mL lyophilized NCL-CD8-4B11 F P (HIER) W
7 mL Bond ready-to-use PA0183 P (HIER)
The CD8 molecule is composed of two chains and has a molecular weight of
32 kD. It is found on a T cell subset of normal cytotoxic/suppressor cells
which make up approximately 20 to 35 percent of human peripheral blood
lymphocytes. The CD8 antigen is reported to be detected on natural killer
cells, 80 percent of thymocytes, on a subpopulation of 30 percent of
peripheral blood null cells and 15 to 30 percent of bone marrow cells.
Refer to page 19 for the Bond ready-to-use format.
/72
For detailed information on all products please visit our website:
www.leica-microsystems.com
Reference Range
Reference Range
Large T cell immunoblastic lymphoma: immunohistochemical staining for CD8 antigen using
NCL-CD8-295. Note intense membrane staining of T lymphocytes. Paraffin section.
CD9 (Motility-Related Protein-1)
Clone 72F6
1 mL lyophilized NCL-CD9 F P (HIER)
CD9 antigen is a 24 to 27 kD glycoprotein expressed on the surface of
developing B lymphocytes, platelets, monocytes, eosinophils, basophils,
stimulated T lymphocytes and by neurons and glial cells in the peripheral
nervous system. It belongs to a family of membrane proteins termed
tetraspanins which transverse the membrane four times. In pre-B cells and
platelets, CD9 antigen regulates cell activation and aggregation possibly
through an association with the integrin CD41/CD61 (GPIIb/GPIIIa). It also
regulates cell motility in a variety of cell lines and appears to be an
important regulator of Schwann cell behavior in the peripheral nervous
system. In melanoma and breast cancer, CD9 antigen expression has been
reported to occur predominantly on primary, non-metastatic tumors.
Human fibroadenoma: immunohistochemical staining for CD9 antigen using NCL-CD9.
Note intense membrane staining of tumor cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.