Labelling Review row-Online

BL-CAM (CD22)
Clone FPC1
1 mL, 0.1 mL lyophilized NCL-CD22-2 P (HIER)
See also CD22 (BL-CAM) on page 75.
Blood Coagulation Factor XIIIa
(Factor XIIIa)
Clone E980.1
1 mL, 0.1 mL lyophilized NCL-FXIIIa P (HIER)
7 mL Bond ready-to-use PA0449 P (HIER)
See also Factor XIIIa (Blood Coagulation Factor XIIIa) on page 109.
BM1 (Myeloid Marker)
Clone BM-1
1 mL lyophilized NCL-BM1 FP
NCL-BM1 is reactive with a 183 kD myeloid-specific DNA-binding protein
expressed in human myeloid precursor cells. The DNA-binding protein is
reported to be found in the cytoplasm and nucleus of normal and malignant
myeloid cells. Unlike other markers, the antigen designated BM-1 appears to
be restricted in its reactivity to M2 and M3 acute myelogenous leukemia
(AML) subtypes.
BM2 (Myeloid Marker)
Clone BM-2
1 mL lyophilized NCL-BM2 F P (HIER)
NCL-BM2 is reactive with a cytoplasmic antigen expressed in human
granulocytes. Markers of myeloid cells are useful in the identification of
different levels of cellular differentiation.
Product Specific Information
NCL-BM1 and NCL-BM2 react with early precursor and mature forms of
human myeloid cells. NCL-BM2 is useful for the detection of myeloid
leukemias and granulocytic sarcomas.
Bone Morphogenetic Protein 4
Clone 3H2
1 mL, 0.1 mL lyophilized NCL-BMP4 PW
Bone morphogenetic protein 4 (BMP4) is one of nine structurally related
BMPs belonging to the transforming growth factor beta (TGF-beta) superfamily
of secreted proteins. Mature BMP4 is a dimer that binds to a
multimeric transmembrane receptor with serine/threonine kinase activity.
Although BMP4 was discovered because it stimulates bone formation in
adult mammals, it has important roles as a signalling molecule in embryonic
tissues including the developing central and peripheral nervous system,
musculature and skeleton. It also participates in a signalling pathway found
in insects and worms. BMP4 is implicated in fibrodysplasia ossificans
progressive. It is reported to be uniquely overexpressed in lymphoblastoid
cells and preosseous fibroproliferative lesions. BMPs 1 to 6 are also
reported to be expressed in prostatic adenocarcinomas with BMPs 1 to 5
also expressed in both benign and prostatic hyperplasia and ocular
melanoma.
Western blot: detection of bone morphogenetic protein 4 (21 kD) using NCL-BMP4. Lane A,
molecular weight markers. Lane B, Saos-2 cell line immunoblotted with NCL-BMP4.
Bromodeoxyuridine
Clone 85-2C8
1 mL lyophilized NCL-BrdU FP
5-Bromodeoxyuridine (BrdU) is an analogue of thymidine. BrdU can be
introduced to *live proliferating cells which in turn incorporate BrdU into the
DNA during S phase, prior to cell division. Immunocytochemical staining for
BrdU is an accurate method for measuring cell proliferation and detects
nucleated cells from different animal species which have incorporated BrdU
in place of thymidine into their DNA. The detection of BrdU incorporation
can be used to determine the proliferative response of cells to mitogenic
stimuli and to monitor the effects of various treatments on tumors.
*Protocol included with product indicates a requirement to incubate fresh
tissue fragments with bromodeoxyuridine before fixation and embedding
followed by sectioning and finally antibody incubation NCL-BrdU via
immunohistochemistry.
Normal human colon biopsy incubated with BrdU and stained using NCL-BrdU. Note intense
nuclear staining of a small proportion of crypt epithelial cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies