Labelling Review row-Online

p53 Protein
Clone IMX25
1 mL lyophilized NCL-p53-505 P (HIER) W
7 mL Bond ready-to-use PA0057 P (HIER)
p53 protein plays a vital role in suppressing the development of cancer. The
accumulation of p53 protein in response to DNA damage in vitro is well
established and appears to induce growth arrest and apoptosis by the
transcriptional regulation of other genes. In irradiated mice, p53 protein
accumulates in splenocytes, thymocytes and osteocytes, though not in
hepatocytes. Mouse T3T3 cells express high levels of phenotypically
characteristic wild type p53 protein which carries two missense mutations.
The range of antigenic sites in mouse p53 protein and human p53 protein is
very similar.
Product Specific Information
NCL-p53-505 is raised to the same recombinant mouse p53 protein as the
polyclonal, NCL-p53-CM5p. It reacts with rat and mouse p53 protein.
Refer to page 38 for the Bond ready-to-use format.
Mouse T3T3 cells: immunohistochemical staining for p53 mouse protein using NCL-p53-505.
Note intense nuclear staining of a proportion of T3T3 cells. Paraffin section.
p53 Protein (1801)
Clone PAb 1801
1 mL, 0.1 mL lyophilized NCL-p53-1801 F P (HIER) W C
The gene for p53 is located on chromosome 17p, a frequent site of allelic
loss in many tumors. It has been reported that a high proportion of breast
and colon carcinomas show immunostaining for p53 protein and expression
of p53 protein.
Product Specific Information
Clone PAb 1801 recognizes both wild type and mutant forms of human p53
protein under denaturing and non-denaturing conditions.
Human colonic adenocarcinoma: immunohistochemical staining for p53 protein using
NCL-p53-1801. Note intense nuclear staining of tumor cells. Paraffin section.
p53 Protein (BP53-12)
Clone BP53-12
1 mL lyophilized NCL-p53-BP FPW
p53 protein plays a vital role in suppressing the development of cancer. The
accumulation of p53 protein in response to DNA damage in vitro is well
established and appears to induce growth arrest and apoptosis by the
transcriptional regulation of other genes.
Product Specific Information
Clone BP53-12 recognizes both wild type and mutant forms of human p53
protein under denaturing and non-denaturing conditions. The heat induced
epitope retrieval technique may improve staining in some cases.
Human breast carcinoma: immunohistochemical staining for p53 protein using NCL-p53-BP.
Note intense nuclear staining of tumor cells. Paraffin section.
p53 Protein (CM1)
Polyclonal
0.2 mL lyophilized NCL-p53-CM1 F P (HIER) W
Mutation of the p53 protein may represent the commonest genetic event in
human malignancy. In colonic tumors, p53 protein has been reported to be
overexpressed in 47 percent of carcinomas and only 9 percent of adenomas.
No expression has been reported in normal mucosa.
Product Specific Information
This polyclonal antibody recognizes both wild type and mutant forms of
human p53 protein under denaturing and non-denaturing conditions. NCLp53-CM1
is less sensitive to overfixation than clone DO-7.
Axillary lymph node infiltrated by metastatic breast carcinoma: immunohistochemical staining
for p53 protein using NCL-p53-CM1. Note intense nuclear staining of a proportion of tumor
cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies