Labelling Review row-Online

Human Herpesvirus (type 8) Probe
(Fluorescein-Conjugated)
50 Tests, 10 Tests liquid probe NCL-HHV8 P
Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma associated
herpesvirus, is one of the eight known human herpes viruses and belongs to
the Gammaherpes virinae, the same subfamily as Epstein-Barr virus. HHV8
has a large double strand DNA genome that carries a complement of over 85
open reading frames.
Product Specific Information
NCL-HHV8 is a cocktail of fluorescein-labelled oligonucleotide probes
contained in 1 mL of hybridization solution, designed to hybridize with a small
transcript, designated T1.1 mRNA, which accumulates in the nuclei of
infected cells.
Human Kaposi's sarcoma: in situ hybridization for human herpesvirus (type 8) (HHV8) mRNA
using NCL-HHV8. Note intense staining of HHV8-infected cells. Paraffin section.
Kappa/Lambda Probes
(Fluorescein-Conjugated)
2 × 25 Tests,2x5Tests liquid probes NCL-KAP/LAM P
Immunoglobulins are polypeptides that consist of heavy and light protein
chains. There are two classes of light chain: kappa and lambda. The ratio of
kappa chains to lambda chains varies in a species-specific fashion. In
humans about 60 percent of light chains are kappa. However, in any
individual immunoglobulin molecule the light chains will be either kappa or
lambda, never a mixture. B cells contain kappa or lambda mRNA.
Product Specific Information
NCL-KAP/LAM consists of two sets of fluorescein-conjugated kappa and
lambda oligonucleotide probes provided in two separate vials, each
containing 0.5 mL of hybridization solution, sufficient for the in situ
hybridization staining of 25 kappa and 25 lambda preparations, respectively.
Human low grade mucosa associated lymphoma of thyroid: in situ hybridization for lambda
mRNA using the lambda probe. Paraffin section.
RUO
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Poly d(T) Probe (Fluorescein-
Conjugated)
50 Tests liquid probe NCL-POLYd(T) P
The precursors of mRNA are transcribed from DNA by RNA polymerase II
and are known as heterogenous nuclear RNA (hnRNA). Enhanced stability is
conferred to 70 to 90 percent of these transcriptions by the addition of
5' methyl caps and 3' tails of approximately 200 adenyl residues. Following
these reactions, most hnRNA is spliced to remove non-coding intron
sequences to produce mRNA. Due to the destruction of RNases by formalin
fixation, polyadenylated mRNA sequences are conserved in routine paraffin
wax preparations, only when they have been fixed promptly. This can be
readily demonstrated using labelled polythymidine (poly d(T)) probes.
Detection of poly A tails provides a way of monitoring the translational
activity of cells and assessing the relative preservation of mRNA in tissue
preparations.
Product Specific Information
NCL-POLYd(T) consists of fluorescein-labelled oligonucleotide for the
detection of polyadenylated mRNA sequences contained in 1 mL of hybridization
solution.
Proteinase K
500 μg lyophilized enzyme NCL-PK P
Product Specific Information
NCL-PK is effective for the digestion of proteins on tissue sections, as a
pre-treatment, to aid in the preparation of mRNA and its detection by in situ
hybridization methods using oligonucleotide probes from Leica
Microsystems.
Universal ISH Detection Kit
100 Tests kit NCL-ISH-D P
Product Specific Information
RUO
The Universal ISH Detection Kit from Leica Microsystems is intended for the
detection of bound fluorescein-conjugated oligonucleotide probes. The ISH
Detection Kit comprises affinity-purified rabbit F(ab') anti-fluorescein
isothiocyanate conjugated to alkaline phosphatase, 5-bromo-4-chloro-3indolyl
phosphate (BCIP) and nitroblue tetrazolium (NBT) and levamisole
hydrochloride.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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ISH Reagents/Probes