Labelling Review row-Online

Mesothelin
Clone 5B2
1 mL lyophilized NCL-MESO F P (HIER)
1 mL liquid NCL-L-MESO F P (HIER)
7 mL ready-to-use RTU-MESO F P (HIER)
7 mL Bond ready-to-use PA0373 P (HIER)
Mesothelin is a glycosyl-phosphatidylinositol-linked (GPI) glycoprotein of
40 kD present on the surface of mesothelial cells, mesotheliomas, epithelial
ovarian cancers and some squamous cell carcinomas. It is synthesized as a
69 kD precursor which is enzymatically processed into an N-terminal
secreted form of 30 kD and the GPI-linked membrane-bound form of 40 kD.
The secreted form is identical to the megakaryocyte potentiating factor, but
it is the GPI-linked membrane-bound form which has generated interest.
Mesothelin is abundantly expressed in the kidney and in occasional
epithelial cells of the trachea, tonsil and fallopian tube. The function of
mesothelin is unclear but it may have a role in cellular adhesion. Mesothelin
is reported to be abundant in the normal mesothelial cells from which
malignant mesotheliomas and ovarian cystadenocarcinomas are derived.
Refer to page 33 for the Bond ready-to-use format.
Human mesothelioma: immunohistochemical staining for mesothelin using NCL-MESO.
Note intense membrane staining of tumor cells. Paraffin section.
Microphthalmia Transcription Factor
(MITF)
Clone 34CA5
1 mL lyophilized NCL-MITF F P (HIER)
1 mL, 0.1 mL liquid NCL-L-MITF F P (HIER)
Microphthalmia transcription factor (MITF) gene product, a nuclear
transcription factor of the basic-helix-loop-helix type, is thought to play a
role in the regulation of genes encoding the enzymes necessary for
melanogenesis. These include tyrosinase, TRP-1 and TRP-2. MITF is critical
for the embryonic development and postnatal viability of melanocytes. The
melanocyte-specific isoform of microphthalmia transcription factor MITF-M,
is reported to be expressed in normal and malignant melanocytes. The other
isoforms, MITF-A, MITF-C and MITF-H, differ structurally at the N-terminus
from MITF-M.
Product Specific Information
Clone 34CA5 is reported to be reactive with the MITF-M isoform.
Human malignant melanoma: immunohistochemical staining for microphthalmia transcription
factor using NCL-L-MITF. Note nuclear staining of melanoma cells. Paraffin section.
Minichromosome Maintenance Protein
Antibodies
Clone CRCT2.1
1 mL, 0.1 mL lyophilized Minichromosome Maintenance
Protein 2 NCL-MCM2 P (HIER)
Clone JCC07
1 mL lyophilized Minichromosome Maintenance
Protein 3 NCL-MCM3 P (HIER)
Clone CRCT5.1
1 mL lyophilized Minichromosome Maintenance
Protein 5 NCL-MCM5 P (HIER) W
Clone DCS-141.1
1 mL lyophilized Minichromosome Maintenance
Protein 7 NCL-MCM7 P (HIER) W
Minichromosome maintenance (MCM) proteins have been reported to play
an essential part in eukaryotic DNA replication. Each of the MCM proteins
have DNA-dependent ATPase motifs in their central domain which are
conserved from yeast to mammals. Both ATPase activity and helicase
activity, which displaces oligonucleotides annealed to single-stranded
circular DNA, are associated with an MCM protein complex. Levels of MCM
proteins generally increase in a variable manner as normal cells progress
from G0 into G1/S phase of the cell cycle. In the G0 phase, MCM2 and MCM5
proteins are reported to be much less abundant than the MCM7 and MCM3
proteins. Therefore, MCM proteins are not present in stoichiometric
amounts and only a proportion of the molecules actively participate in cell
cycle regulation as part of MCM complexes. Oncoprotein E6 of the human
papillomavirus (HPV), associated with cervical cancer (HPV-16 and -18),
degrades the tumor suppressor protein p53, but also seems to have p53independent
transforming functions. E6 was reported to bind to the Cterminal
region of the human MCM7 protein causing chromosomal
abnormalities in human cells expressing E6 proteins of oncogenic HPVs.
Product Specific Information
NCL-MCM2, NCL-MCM3, NCL-MCM5 and NCL-MCM7 are specific for
minichromosome maintenance proteins 2, 3, 5 and 7, respectively.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies