Labelling Review row-Online

Primary Antibodies
Parathyroid Hormone
Clone 105G7
1 mL, 0.1 mL lyophilized NCL-PTH-488 P
The parathyroid glands are small, oval, endocrine glands closely associated
with the thyroid gland. The parathyroid glands regulate serum calcium and
phosphate levels via parathyroid hormone (parathormone). Parathyroid
hormone raises serum calcium levels directly, by increasing the rate of
osteoclastic reabsorption and promoting breakdown of the bone matrix, and
indirectly, by increasing the renal tubular reabsorption of calcium ions and
inhibiting the reabsorption of phosphate ions from the glomerular filtrate,
and finally, by promoting the absorption of calcium from the small intestine.
Parathyroid hormone is the most important regulator of blood calcium levels
and is essential to life, whereas calcitonin appears only to provide a
complementary mechanism for fine adjustment. Chief cells are the most
abundant cells in the parathyroid gland and are responsible for the
secretion of parathyroid hormone. Antibodies to parathyroid hormone
together with antibodies to thyroglobulin are useful in studies to differentiate
parathyroid-derived lesions from thyroid-derived lesions.
Human parathyroid: immunohistochemical staining for parathyroid hormone using NCL-PTH-
488. Note cytoplasmic staining of chief cells. Paraffin section.
Parvalbumin (Alpha)
Clone 2E11
1 mL lyophilized NCL-PARVALBUMIN P (HIER)
Alpha and beta parvalbumins are low molecular weight, water-soluble,
calcium-binding proteins. The protein is found in a subset of fast-spiking
inhibitory GABAergic interneurons with a Ca2+ buffering capacity that
reduces the Ca2+ -dependent K + outward current. Unlike other Ca2+ binding
proteins, parvalbumin-containing neurons appear to co-localize only with
corticotropin-releasing factor and not with other neuropeptides associated
with GABA such as somatostatin, neuropeptide Y and cholecystokinin.
Neurons which contain parvalbumin appear to be resistant to ischemia,
epilepsy and N-methyl-D-aspartate receptor agonists due to their ability to
buffer increase in intracellular calcium. Alpha and beta parvalbumins are
reported to be expressed in different human tissues with the alpha form
highly expressed in extracts of human cerebellum, weakly in kidney and not
in skeletal muscle, thymus, lung, placenta, heart, liver and diaphragm. The
beta form of parvalbumin has been detected only in preterm placenta. These
expression patterns differ significantly between human and rodent species
with these differences also reflected with some members of the S-100 family
of Ca2+ binding proteins. Within the cerebellum, alpha parvalbumin is
reported to be localized to Purkinje, basket, stellate and Golgi cells. In cases
of spinocerebellar ataxia-1 (SCA-1), the number of Purkinje cells expressing
alpha parvalbumin is reported to be much reduced, which may reflect
biochemical changes preceding Purkinje degeneration.
Product Specific Information
NCL-PARVALBUMIN does not detect parvalbumin in preterm placenta
indicating its specificity for the alpha form of this protein.
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Human brain, cerebellum: immunohistochemical staining for alpha parvalbumin using NCL-
PARVALBUMIN. Note cytoplasmic staining of Purkinje, basket and stellate cells. Paraffin
section.
Parvovirus B19
Clone R92F6
1 mL, 0.1 mL lyophilized NCL-PARVO FP
Parvovirus B19 is a small, single-stranded DNA virus which causes
erythema infectiosum also known as ‘slapped cheek syndrome'. Clinically,
this is a febrile disease in children, often epidemic, with a facial
maculopapular rash causing flushed cheeks. In individuals with erythrocyte
abnormalities, such as sickle cell anaemia, Parvovirus B19 can cause
hemolytic complications where the virus replicates in bone marrow cells
and inhibits erythropoiesis. Parvovirus B19 has also been implicated with
spontaneous abortion in humans.
Product Specific Information
NCL-PARVO is specific for the viral antigens, VP1 (84 kD) and VP2 (58 kD).
Human fetal lung, post-mortem tissue: immunohistochemical staining for Parvovirus B19 using
NCL-PARVO. Note intense staining of infected cells within the capillaries. Paraffin section.
Pax-5
Clone 1EW
1 mL liquid NCL-L-PAX-5 P (HIER) W
7 mL Bond ready-to-use PA0552 P (HIER)
Pax genes are a family of developmental control genes that encode nuclear
transcription factors and have been implicated in the control of mammalian
development. PAX-5 is a B cell specific transcription factor that is
expressed in pro B cells, pre-B and mature B cells, and subsequently in all
stages of B cell development until the plasma cell stage in which it is
downregulated.
Product Specific Information
The use of H2O2 to block endogenous peroxidase has been shown to have a
detrimental effect on the epitope recognized by Clone 1EW It is, therefore,
critical that blocking with H2O2 should be carried out after application of the
primary antibody with solutions of no greater than 3 percent, otherwise
staining intensity will be reduced.
Refer to page 39 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.