Labelling Review row-Online

P-selectin (CD62P)
Clone C34
1 mL lyophilized NCL-CD62P-367 P (HIER)
The CD62P antigen (140 kD), also known as P-selectin, mediates the
interaction of activated platelets with neutrophils and monocytes and is
responsible for the rolling attachment of neutrophils to activated
endothelium. CD62P antigen binds to the carbohydrate structures Sialyl-
Lewis x on neutrophils and to galactosyl ceramides on neutrophils and tumor
cells. A soluble CD62P antigen inhibits the integrin-mediated adhesion of
activated neutrophils to endothelium.
Human umbilical cord, blood vessel: immunohistochemical staining for CD62P antigen
(P-selectin) using NCL-CD62P-367. Note intense staining of platelets. Paraffin section.
PTEN
Clone 28H6
1 mL, 0.1 mL lyophilized NCL-PTEN P (HIER)
PTEN, also known as MMAC1 or TEP1, is a tumor suppressor gene which
encodes a multifunctional phosphatase which is expressed almost ubiquitously
and regulates the cell cycle, apoptosis and possibly cell adhesion.
PTEN also interacts with the focal adhesion kinase (FAK) reducing FAK
phosphorylation affecting cell adhesion, spreading and recognition. In
addition, PTEN may prevent resistance to apoptosis by dephosphorylating
phosphatidylinositol 3, 4, 5-triphosphate. Deletions and mutations to PTEN
occur in a range of cancers including breast cancer, malignant melanoma,
endometrial carcinoma, bladder carcinoma, small cell lung carcinoma and
endometrial ovarian cancer. Germline mutations of PTEN are found in
Cowden syndrome which leads to a predisposition for breast and thyroid
cancers. Loss of PTEN gene expression is reported to occur in both breast
and prostate cancer.
Human malignant melanoma: immunohistochemical staining for PTEN using NCL-PTEN.
Note nuclear staining in a proportion of malignant cells. Paraffin section.
Renal Cell Carcinoma
Marker
Clone 66.4.C2
1 mL, 0.1 mL lyophilized NCL-RCC P (Enzyme)
In the normal kidney, a 200 kD glycoprotein is localized within the brush
border of the pars convoluta and pars recta segments of the proximal renal
tubule and on the luminal surface of Bowman's capsule adjoining the
outgoing proximal tubule. The glycoprotein, gp200, is also reported to be
expressed on the luminal surface of breast lobules and ducts, the luminal
surface of the epididymal tubular epithelium and within the colloid of thyroid
follicles. Reports indicate gp200 antigen to be expressed in approximately
93 percent of primary and 84 percent of metastatic renal cell carcinomas.
Product Specific Information
NCL-RCC is specific for a proximal nephrogenic renal antigenic site on the
carbohydrate domain of gp200.
Human renal cell carcinoma: immunohistochemical staining for gp200 using NCL-RCC. Note
cytoplasmic staining of tumor cells. Paraffin section. Section supplied courtesy of Dr Mouza
Abdulla A. Al-Sharhan.
Respiratory syncytial virus
Clone 5H5N, G12 2 , 5A6, IC3 cocktail
1 mL lyophilized NCL-RSV3 F P (HIER) I
Reference Range
Respiratory syncytial virus (RSV) is the most important respiratory pathogen
of childhood and is responsible for approximately 50 percent of all cases of
bronchiolitis and 25 percent of all cases of pneumonia during the first few
months of life. Approximately one percent of babies who develop an RSV
infection between two and six months die, particularly those with congenital
heart defects, bronchopulmonary dysplasia, low birth weight or
immunodeficiency. The virus is also associated with significant lower
respiratory disease in elderly and immunosuppressed individuals in whom
mortality rates may be high. Multiple types and subtypes of RSV cocirculate
in the population.
Product Specific Information
NCL-RSV3 is a cocktail of four antibodies. NCL-RSV3 does not cross-react
with Parainfluenza virus types 1, 2, 3 and 4b, Adenovirus, Mumps virus,
Measles virus, Influenza virus types A and B, Poliovirus types 1, 2 and 3,
Coxsackie B4 virus, echovirus 19, Varicella-zoster virus, Cytomegalovirus and
Herpes simplex virus types 1 and 2.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies