Labelling Review row-Online
Labelling Review row-Online
Labelling Review row-Online
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Primary Antibodies<br />
Beta-2-Microglobulin<br />
Polyclonal<br />
1 mL lyophilized NCL-B2Mp P (Enzyme) O<br />
Beta-2-microglobulin, a single polypeptide chain of molecular weight 11.6<br />
kD, is present on the surface of most nucleated cells and its expression may<br />
be decreased or lost in malignancy. Beta-2-microglobulin is the major<br />
constituent of a subtype of secondary amyloidosis which is associated with<br />
long term hemodialysis. Clinical and pathological features of this disease<br />
have been characterized. Spontaneous fractures and destructive arthropathies<br />
(articular swelling and pain in an oligoarticular distribution, along<br />
with effusions in large joints) have been related to amyloid deposition.<br />
Amyloid has been implicated in most clinical complaints of beta-2microglobulin-related<br />
amyloid arthropathy where it is found in synovial<br />
biopsies taken from the involved joints.<br />
Product Specific Information<br />
NCL-B2Mp is also effective in ELISA techniques.<br />
Beta Amyloid<br />
Clone 6F/3D<br />
1 mL lyophilized NCL-B-Amyloid FP<br />
Beta amyloid is an extracellular filamentous protein deposit found in the<br />
brain. It is the major protein component of amyloid cores and neuritic<br />
plaques and is also found as a deposit in neurofibrillary tangles. In man,<br />
Alzheimer's disease is the most common cause of senile dementia and is<br />
characterized by abnormal filamentous protein deposits in the brain. Beta<br />
amyloid deposits are also detected in Lewy body dementia, Down's<br />
syndrome, amyloidosis (Dutch type) and in the Guam Parkinson-Dementia<br />
complex.<br />
Product Specific Information<br />
Pretreatment of tissue sections with 98 to 100 percent formic acid is<br />
recommended when using NCL-B-Amyloid.<br />
Human brain, Alzheimer's disease: immunohistochemical staining for beta amyloid protein<br />
using NCL-B-Amyloid. Note intense staining of senile plaques. Paraffin section.<br />
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For detailed information on all products please visit our website:<br />
www.leica-microsystems.com<br />
Beta-Catenin<br />
Clone 17C2<br />
1 mL, 0.1 mL lyophilized NCL-B-CAT F P (HIER) W<br />
7 mL Bond ready-to-use PA0083 P (HIER) New!<br />
The catenins, (alpha, beta and gamma) are cytoplasmic proteins which bind<br />
to the highly conserved tail of the E-cadherin molecule. Beta-catenin is a<br />
component of the adherens junction, a multiprotein complex which supports<br />
Ca2+ -dependent cell to cell contact which in itself is critical for adhesion,<br />
signal transmission and for anchoring the actin cytoskeleton. Beta-catenin’s<br />
role is as a transcription effector of the wnt-signalling pathway. Immunohistochemistry<br />
is the best way to demonstrate nuclear expression of betacatenin<br />
and wnt-pathway activation. This aberrant expression is observed in<br />
human tumorigenesis and especially in colorectal cancer.<br />
Refer to page 15 for the Bond ready-to-use format.<br />
Human endometrial adenocarcinoma: immunohistochemical staining for beta-catenin using<br />
NCL-B-CAT. Note membrane staining of tumor cells. Paraffin section.<br />
Beta-Dystroglycan<br />
Clone 43DAG1/8D5<br />
1 mL, 0.1 mL lyophilized NCL-b-DG FWE<br />
Dystrophin associated glycoproteins (DAGs) are a complex of at least seven<br />
proteins involved in the attachment of dystrophin to muscle membranes. The<br />
biological significance of this dystrophin/glycoprotein complex is not fully<br />
understood, but it appears to form an essential linkage between actin on the<br />
inside of the muscle fiber and muscle laminin in the basal lamina which<br />
surrounds the fiber. Beta-dystroglycan spans the muscle membrane and it<br />
has been suggested that it is the member of the complex which binds<br />
directly to dystrophin. Labeling of beta-dystroglycan may be reduced in<br />
some forms of muscular dystrophy where another component eg dystrophin<br />
or laminin, is directly affected. Labeling with an antibody to beta-spectrin to<br />
monitor membrane integrity, is an essential immunohistochemical control.<br />
Western blot: detection of human beta-dystroglycan (43 kD) using NCL-b-DG. Lane A,<br />
molecular weight markers. Lane B, human skeletal muscle extract immunoblotted with<br />
NCL-b-DG.<br />
Products in this catalog are subject to regulatory approval.<br />
This catalog is not for use in the USA.