Labelling Review row-Online

Human Chorionic Gonadotrophin (alpha)
Clone 4E12
1 mL lyophilized NCL-HCG-alpha F P (HIER)
The human chorionic gonadotrophin alpha (hCGa) gene has now been
identified as an estrogen receptor alpha (ERa) responsive gene in breast
cancer cells. It encodes the common alpha subunit of the four secreted
glycoprotein hormones, hCG, LH, FSH and TSH. The common alpha chain
and the hormone-specific beta chains have molecular weights of 14 kD and
17 kD, respectively. hCGa is expressed as part of hCG in normal placenta
and as part of LH, FSH and TSH in the pituitary gland. hCGa mRNA is
reported to be detected in normal pregnant women and in the peripheral
blood mononuclear cells of patients with trophoblastic disease. Additionally,
hCG is reported to be detected in seminomatous and non-seminomatous
testicular cancers as well as in its free alpha and beta subunits.
Independent studies suggest that approximately half of ERa positive breast
cancers express hCGa.
Normal human placenta: immunohistochemical staining for human chorionic gonadotrophin
alpha using NCL-HCG-alpha. Note cytoplasmic staining of syncytiotrophoblasts. Paraffin
section.
Human Chorionic Gonadotrophin (beta)
Polyclonal
1 mL lyophilized NCL-HCGp F P (Enzyme)
7 mL Bond ready-to-use PA0014 P (HIER)
Human chorionic gonadotrophin (hCG) is a glycoprotein hormone produced
by trophoblastic cells of the placenta beginning 10 to 12 days after
conception. Maintenance of the fetus in the first trimester of pregnancy
requires the production of hCG, which binds to the corpus luteum of the
ovary which is stimulated to produce progesterone which in turn maintains
the secretory endometrium. hCG is composed of two subunits, alpha and
beta. The alpha subunit of hCG is identical to the subunit of luteinising
hormone, thyroid stimulating hormone and follicle stimulating hormone. The
common alpha chain and the hormone-specific beta chains have molecular
weights of 14 kD and 17 kD, respectively. The hCG beta-subunit is unique in
the family of beta-containing glycoprotein hormones in that it contains an
extension of 29 amino acids at its COOH end. It is believed that the Cterminal
region of the HCG-beta subunit plays a role in the intracellular
behavior of the heterodimer.
Product Specific Information
NCL-HCGp was raised to the isolated beta-chain of human chorionic
gonadotrophin and reacts with placental trophoblasts. NCL-HCGp shows a
slight cross-reaction with luteinising hormone and may, therefore, stain
basophil cells in the pituitary.
Refer to page 31 for the Bond ready-to-use format.
Human Gastric Mucin (HGM-45M1)
Clone 45M1
1 mL, 0.1 mL lyophilized NCL-HGM-45M1 F P (HIER)
Many of the cancer associated antigens have been identified as mucin
antigens. The expression of these antigens are associated with the earliest
steps in mucin glycosylation which in turn is associated with several
diseases. Human Gastric mucin is found on the surface of gastric epithelium
of the normal gastrointestinal tract. The “gastric mucins” include Muc-1,
Muc-5AC and Muc-6 glycoproteins.
Product Specific Information
NCL-HGM-45M1 recognizes the mucin epitope located in the peptide core of
gastric mucin, fulfilling a similar function to the antibody, NCL-MUC-1-CORE.
Thiol reduction (using 2-mercaptoethanol) completely destroys this epitope,
which is partially lost following trypsin proteolysis but is stable upon
periodate oxidation.
Human Growth Hormone (HGH)
Polyclonal
0.25 mL lyophilized NCL-HGH FP
7 mL Bond ready-to-use PA0704 P
Growth hormone (GH), somatotropin, is the primary hormone responsible for
regulating overall body growth and is also important in organic metabolism.
It is synthesized by acidophilic or somatotropic cells of the anterior pituitary
gland. Human GH has a molecular weight of 22 kD. GH stimulates growth
indirectly by promoting the liver's production of somatomedins, which act
directly on bone and soft tissue to cause growth. GH exerts direct metabolic
effects on the liver, adipose tissue and muscle. In general, growth hormone
enhances protein synthesis, conserves carbohydrates and uses up fat
stores.
Refer to page 31 for the Bond ready-to-use format.
Human Herpesvirus (type 8)
(latent nuclear antigen)
Clone 13B10
1 mL, 0.1 mL lyophilized NCL-HHV8-LNA P (HIER) W
Human herpesvirus type 8 (HHV8), is the proposed etiological agent of
Kaposi's sarcoma (KS). It is reported that HHV8 has been demonstrated in
KS tissues by immunohistochemistry, in situ PCR and also in situ
hybridization. HHV8 encodes a latent nuclear antigen (LNA) which is the
product of the viral gene orf 73. LNA is capable of forming a complex with
retinoblastoma susceptibility gene product which may be related to its
oncogenic activity. HHV8 has been reported to be expressed in multicentric
Castleman's disease (MCD) and in angioimmunoblastic lymphadenopathies.
The localization of HHV8 in subcapsular spindle cell proliferations, which is
where early intranodal KS begins, and endothelial cells in Castleman's
disease may explain the link between intranodal KS and MCD. In MCD,
HHV8 is reported to be expressed in mantle zone large immunoblastic
B cells.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies