Labelling Review row-Online
Labelling Review row-Online
Labelling Review row-Online
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S-100<br />
Clone S1/61/69<br />
1 mL, 0.1 mL lyophilized NCL-S100 FP<br />
Polyclonal<br />
1 mL lyophilized NCL-S100p FP<br />
1 mL liquid polyclonal NCL-L-S100p FP<br />
7 mL ready-to-use RTU-S100p FP<br />
7 mL Bond ready-to-use PA0900 P (Enzyme)<br />
S-100A and S-100B proteins are two members of the S-100 family of proteins.<br />
S-100A is composed of an alpha and beta chain whereas S-100B is<br />
composed of two beta chains. S-100 protein is reported to be expressed in<br />
neuroectodermal tissue, including nerves and melanocytes. Langerhans<br />
cells in skin and interdigitating reticulum cells in the paracortex of lymph<br />
nodes are also reported to express S-100 protein. It is noteworthy that S-100<br />
protein is highly soluble and may be eluted from frozen tissue during<br />
immunohistochemical procedures.<br />
Product Specific Information<br />
NCL-S100 is specific for the A form of S-100 and so is not recommended for<br />
the staining of neural elements. NCL-S100p, NCL-L-S100p and RTU-S100p<br />
may require enzyme pretreatment in some cases and are also reactive with<br />
S-100 protein from cow, chicken, pig, dog, cat, monkey, horse, mouse and rat.<br />
NCL-S100 may require heat induced epitope retrieval (HIER) in some cases.<br />
Refer to page 40 for the Bond ready-to-use format.<br />
S100A7 (Psoriasin)<br />
Clone 3DC<br />
1 mL liquid NCL-L-S100-A7 P (HIER)<br />
Reference Range<br />
S100A7, also known as psoriasin, is a member of the S-100 gene family of<br />
calcium binding proteins which regulate a variety of intracellular and<br />
extracellular processes. It is largely, although not entirely, reported to be<br />
confined to stratified squamous epithelium. Reports indicate that in breast<br />
cancer, high levels of S100A7 are associated with estrogen and<br />
progesterone receptor negative status and nodal metastasis. It is also<br />
reported to be expressed in abnormal keratinization in squamous cell<br />
carcinoma.<br />
Human malignant melanoma: immunohistochemical staining for S100A7 using NCL-L-S100A7.<br />
Note intense staining of tumor cells. Paraffin section.<br />
Sarcoglycan Antibodies<br />
Clone Ad1/20A6<br />
1 mL, 0.1 mL lyophilized sarcoglycan, alpha (adhalin)<br />
NCL-a-SARC FWE<br />
1 mL liquid sarcoglycan, alpha (adhalin)<br />
NCL-L-a-SARC FWE<br />
Clone �Sarc1/5B1<br />
1 mL, 0.1 mL lyophilized sarcoglycan, beta<br />
NCL-b-SARC FE<br />
1 mL liquid sarcoglycan, beta NCL-L-b-SARC FE<br />
Clone �Sarc3/12C1<br />
1 mL lyophilized sarcoglycan, delta<br />
NCL-d-SARC FW<br />
Clone 35DAG/21B5<br />
1 mL, 0.1 mL lyophilized sarcoglycan, gamma<br />
NCL-g-SARC FE<br />
In normal skeletal muscle, dystrophin, the protein product of the gene which<br />
is defective in Duchenne and Becker muscular dystrophy, is attached to the<br />
muscle membrane via a complex of at least seven proteins (dystrophin<br />
associated glycoproteins, DAGs). Dystrophin-deficient muscle shows a<br />
generalized reduction in DAG labeling. Recent studies have shown that<br />
expression of different members of the dystrophin glycoprotein complex are<br />
altered in several types of muscular dystrophy: the picture is complex and<br />
disease classification is currently under review. For example, individuals<br />
with LGMD2D have mutations in the gene for alpha-sarcoglycan, those with<br />
LGM2E have mutations in the beta-sarcoglycan gene, and those with LGM2F<br />
have mutations in the delta-sarcoglycan gene. Also, many individuals with<br />
severe childhood autosomal recessive muscular dystrophy (SCARMD) show<br />
defective expression in the sarcoglycan subgroup of complex proteins<br />
which includes alpha-sarcoglycan (adhalin) and gamma sarcoglycan. As the<br />
sarcoglycans function together as a subcomplex, individuals with mutations<br />
in any one of the sarcoglycan genes usually show reduced expression for<br />
the whole group, but the reduction may be most severe for the mutated<br />
single protein. Labeling for beta-spectrin is necessary to monitor membrane<br />
integrity.<br />
Western blot: detection of alpha-sarcoglycan (50 kD) using NCL-L-a-SARC. Lane A, molecular<br />
weight markers. Lane B, human muscle extract immunoblotted with NCL-L-a-SARC.<br />
F Frozen I Immunofluorescence E Electron microscopy<br />
P Paraffin C Flow cytometry O Other applications<br />
W Western blotting<br />
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Primary Antibodies