Labelling Review row-Online

Bond
E-Cadherin
Clone 36B5
7 mL Bond ready-to-use PA0387 P (HIER)
For In Vitro Diagnostic Use
E-cadherin is a Ca2+ -dependent, transmembrane cell adhesion molecule. It
plays an important role in the growth, development and the intercellular
adhesion of epithelial cells. Most tumors have an abnormal architecture and
any subsequent loss of adhesiveness is thought to be an important step in
the development of local invasion. E-cadherin may have a role in neoplastic
progression, particularly as a suppressor of invasion. In prostate cancers,
for example, the expression of E-cadherin is reported to be reduced or
absent in comparison with its expression in normal prostate which is
uniformly strong. Reduced expression or absence of E-cadherin in addition
to alpha, beta and gamma-catenin in primary breast carcinomas has also
been reported and these four proteins are associated with the development
of metastases.The main immunohistochemical utility of E-cadherin is to
highlight differential expression of this protein in lobular and ductal
carcinomas.
Also available as a Novocastra concentrate, refer to page 102.
Bowel: immunohistochemical staining with Bond ready-to-use E-Cadherin (36B5) using Bond
Polymer Refine Detection.
Epithelial Membrane Antigen
Clone GP1.4
7 mL Bond ready-to-use PA0035 P (HIER)
For In Vitro Diagnostic Use
Epithelial membrane antigen (EMA), also known as episialin, has a
molecular weight in the range 265 to 400 kD. In normal tissues, EMA is
reported to be expressed in a variety of normal epithelia with the highest
expression reported at the apical portion of the ductal lining cells of
mammary epithelium. A similar pattern of expression has been reported in
other glandular epithelia eg sweat glands, while squamous epithelium
shows an uneven pattern of antigenic expression.
Also available as a Novocastra concentrate, refer to page 105.
Rectal adenocarcinoma: immunohistochemical staining with Bond ready-to-use Epithelial
Membrane Antigen (GP1.4) using Bond Polymer Refine Detection.
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Estrogen Receptor
Clone 6F11
7 mL Bond ready-to-use PA0151 P (HIER)
For In Vitro Diagnostic Use
Estrogen Receptor (6F11) is indicated as an aid in the prediction of prognosis
for breast cancer. Clone 6F11 binds specifically to the Estrogen Receptor
alpha (ER�) antigen in the nuclei of cells in normal tissues that express high
levels of ER; a proportion of endometrial, ovarian and myometrial cells, and
normal breast ductal cells.
The dysregulation of ER� is thought to contribute to breast tumorigenesis.
Presence of ER� in these tumors is related to an improved overall survival
and favorable response to endocrine therapy such as anti-estrogen
Tamoxifen.
Also available as a Novocastra concentrate, refer to page 106.
Breast carcinoma: immunohistochemical staining with Bond ready-to-use Estrogen Receptor
(6F11) using Bond Polymer Refine Detection.
Factor XIIIa
Clone E980.1
7 mL Bond ready-to-use PA0449 P (HIER)
For In Vitro Diagnostic Use
Factor XIIIa, also known as fibrinoligase and fibrin-stabilizing factor, is the
last enzyme generated in the blood coagulation cascade. It is a Ca2+ -
dependent transglutaminase or transamidating enzyme which forms
intermolecular gamma-glutamyl-epsilon-lysine crosslinks between fibrin
molecules resulting in the mechanical stabilization of the fibrin clot and its
resistance to proteolysis. Factor XIIIa may also function to stabilize cell
surface molecules and membranes. Ca2+ -dependent trans-glutaminases
with thiol active centers are widespread in animal tissues and have been
associated with cell proliferation, embryonic development and growth
through the proliferation of mammary stroma and epithelial elements.
Normal mammary stroma, like most collagenous connective tissue contains
resident populations of CD34 positive dendritic interstitial cells and
scattered Factor XIIIa positive collagen-associated dendrophages. Factor
XIIIa has been examined to determine its expression in normal and inflamed
skin. Factor XIIIa positive cells in human skin represent a specific population
of bone marrow dermal dendritic cells, distinct from Langerhans cells which
share some features common to mononuclear phagocytes. In benign skin
conditions such as inflammatory dermatoses eg atopic eczema and
psoriasis, an increased number of factor XIIIa positive cells in the upper
dermis, closely associated with lymphocytes, has been described.
Also available as a Novocastra concentrate, refer to page 109.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.