Labelling Review row-Online

Lymphocyte Activation Gene-3 Protein
(CD223)
Clone 12H6
1 mL lyophilized NCL-LAG-3 P (HIER)
Lymphocyte activation gene-3 (LAG-3) is a member of the immunoglobulin
superfamily. It is a major histocompatability (MHC) class II ligand that is
evolutionary-related to the CD4 molecule and is expressed in activated T
and natural killer (NK) lymphocytes. It is reported that it may play a role in
regulating the evolving immune response. LAG-3 is associated with the CD3/
TCR complex, CD8 and MHC class II molecules where the resultant
supramolecule may arise from an organisation in raft microdomains, a
phenomenon known to regulate early events of T cell activation. LAG-3
expression is reported to be up-regulated by certain cytokines eg IL-2, IL-12
and not by others eg IL-4, IL-6, IL-10, TNF-alpha and -beta and IFN-gamma.
Inflamed human tonsil: immunohistochemical staining for lymphocyte activation gene-3 protein
using NCL-LAG-3. Note membrane and cytoplasmic staining of activated T lymphocytes and
natural killer cells. Paraffin section.
Lysozyme (Muramidase)
Polyclonal
1 mL lyophilized NCL-MURAM P (Enzyme) W
7 mL Bond ready-to-use PA0391 P (Enzyme)
New!
Intracellular muramidase, also known as lysozyme, has been reported to be
expressed in myeloid and monocytic cells, in leukocytes and in myeloproliferative
disorders. Muramidase is also reported to be expressed in
poorly differentiated leukemic monoblasts.
Refer to page 35 for the Bond ready-to-use format.
Human spleen, myeloid leukemia: immunohistochemical staining for muramidase (lysozyme)
using NCL-MURAM. Note intense cytoplasmic staining of myeloid cells. Paraffin section.
Mac-1 (CD11b)
Clone 44
1 mL lyophilized NCL-CD11b F
See also CD11b (Mac-1) on page 73.
Macrophage Marker (LN-5)
Clone LN-5
1 mL lyophilized NCL-LN5 F P (Enzyme)
Different types of lymphomas of true histiocytic origin can be characterized
with various panels of antibodies. Expression of different phenotypes in socalled
true histiocytic lymphoma and malignant histiocytosis has been
observed but all express a group of markers that includes LN5. Hsu SM et al.
American Journal of Pathology. 138(6):1389-404 (1991).
Product Specific Information
Clone LN-5 stains the cytoplasm of macrophages and histiocytes in
hematopoietic organs. The heat induced epitope retrieval (HIER) technique
may enhance staining in some cases.
Human small intestine: immunohistochemical staining for a marker of the histiocyte/
macrophage series using NCL-LN5. Note intense cytoplasmic staining of macrophages.
Paraffin section.
Macrophage Marker (MAC387)
Clone MAC387
1 mL lyophilized NCL-MAC387 F P (Enzyme)
7 mL Bond ready-to-use PA0752 P (HIER)
L1, a member of the S-100 family of proteins, is reported to be found on
neutrophils, monocytes, certain reactive macrophages and squamous
mucosal epithelia.
Product Specific Information
Clone MAC387 is reported to be specific for the leucocyte antigen L1.
Refer to page 32 for the Bond ready-to-use format.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies