Labelling Review row-Online

Primary Antibodies
Alpha-Catenin
Clone 25B1
1 mL lyophilized NCL-A-CAT F P (HIER) W
Alpha-catenin, which shows some homology with vinculin, appears to play a
role in tumor invasion and metastasis through the dysfunction of E-cadherin.
Normal epithelium of the esophagus, stomach and colon express alphacatenin
strongly, without exception. However, in primary tumors of these
tissues its expression is frequently reduced. It has been suggested that
some human cancer cells may have impaired E-cadherin-mediated cell
adhesiveness as a result of the downregulation of alpha-catenin expression.
Abnormalities in the expression of alpha-catenin seem to associate with
malignant cellular features and disease progression in prostate cancer. In
one study of several tumor-related proteins in breast cancers, it was shown
that alpha and beta-catenins could be re-expressed in metastatic tissue. Reexpression
of these adhesion molecules by tumor cells after release from
the primary site may be important and perhaps necessary for cells to adhere
in remote organs.
Human appendix: immunohistochemical staining for alpha catenin using NCL-A-CAT.
Note intense membrane staining of the mucosal epithelial cells. Paraffin section.
Alpha Fetoprotein
Clone C3
1 mL, 0.1mL lyophilized NCL-AFP FP
7 mL Bond ready-to-use PA0963 P
Alpha fetoprotein (AFP) is an oncofetal antigen of 70 kD found in body fluids
which if detected in high concentrations has clinical implications. AFP is
expressed in fetal liver but is not present under normal circumstances in
healthy adult tissues. It is reported to be expressed in a proportion of germ
cell tumors, with high frequency in yolk sac tumors.
Refer to page 14 for the Bond ready-to-use format.
Human fetal liver: immunohistochemical staining for alpha fetoprotein using NCL-AFP.
Note cytoplasmic staining of hepatocytes. Paraffin section.
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Alpha-Internexin
Clone 2E3
1 mL lyophilized NCL-A-INTER P (HIER)
Alpha-internexin is a 66 kD protein which shares several characteristics
with intermediate filament proteins. In rats, alpha-internexin is often copurified
with intermediate filaments from spinal cord and optic nerve. The
protein is axonally transported together with the neurofilament triplet
proteins (68, 160 and 200 kD) along the length of the optic nerve. Although
the distribution of alpha-internexin is similar to the light component
neurofilament (68 kD), its distribution in rat embryo is far more extensive in
the early stages of development. There is also evidence that expression of
alpha-internexin is heavily upregulated in damaged neurons.
Human brain, basal ganglia: immunohistochemical staining for alpha-internexin using
NCL-A-INTER. Note intense cytoplasmic staining of white matter tracts. Paraffin section.
Alpha-Methylacyl-CoA Racemase
(AMACR, p504s)
Clone EPUM1 New!
1 mL, 0.1 mL liquid NCL-L-AMACR P (HIER)
Alpha-methylacyl-CoA racemase (AMACR), also known as p504s, is a
mitochondrial and peroxisomal enzyme that is involved in bile acid
biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is
essential in lipid metabolism, and is expressed in normal liver (hepatocytes),
kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression
has also been found in lung (bronchial epithelial cells) and colon (colonic
surface epithelium). Expression is granular and cytoplasmic. AMACR
expression can also be found in hepatocellular carcinoma and kidney
carcinoma. Past studies have also shown that AMACR is expressed in
various colon carcinomas (well, moderately and poorly differentiated) and
over expressed in prostate carcinoma.
Human prostatic adenocarcinoma: immunohistochemical staining for alpha-methylacyl-CoA
racemase (AMACR, p504S) using NCL-L-AMACR. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.