Labelling Review row-Online

Primary Antibodies
Human tonsil: immunohistochemical staining for CD72 antigen using NCL-CD72.
Note membrane staining of B lymphocytes. Paraffin section.
CD79a
Clone 11E3
1 mL, 0.1 mL lyophilized NCL-CD79a-225 F P (HIER)
1 mL liquid NCL-L-CD79a-225 F P (HIER) C
7 mL Bond ready-to-use PA0192 P (HIER)
Clone 11D10
1 mL, 0.1 mL lyophilized NCL-CD79a-192 F P (HIER) C
1 mL liquid NCL-L-CD79a-192 F P (HIER)
7 mL ready-to-use RTU-CD79a-192 F P (HIER)
Clone 11E3 was developed to produce superior staining on paraffin sections.
The CD79 complex is a disulfide-linked heterodimer which is non-covalently
associated with membrane-bound immunoglobulins on B cells. This complex
of polypeptides and immunoglobulin constitute the B cell antigen receptor.
The two components of this complex are designated CD79a and CD79b. The
CD79a antigen is reported to first appear at the pre-B cell stage, early in
maturation, and persist until the plasma cell stage where it is found as an
intracellular component. The CD79a antigen is reported to be expressed in
the majority of acute leukemias of precursor B cell type, B cell lines, B cell
lymphomas and in some myelomas. It is not present in myeloid or T cell lines.
Refer to page 24 for the Bond ready-to-use format.
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Human large cell lymphoma: immunohistochemical staining for CD79a antigen using
NCL-CD79a-225. Note membrane staining of tumor cells. Paraffin section.
CD79b
Clone JS01
1 mL, 0.1 mL liquid NCL-L-CD79b P (HIER)
CD79b, also known as B29 and Ig-� is thought to function in the cellular
activation and signalling that occurs when surface immunoglobulin (Ig) on B
cells binds antigen or becomes cross-linked by anti-Ig antibody. This
function occurs with the formation of a membrane signalling complex that is
associated with Ig at the surface of B cells. CD79b, together with CD79a,
forms the B cell antigen receptor (mlg) complex. CD79b expression is
reported to be found in 80 to 90 percent of mature B cell neoplasms, with the
exception of chronic lymphocytic leukemias.
Human tonsil: immunohistochemical staining for CD79b using NCL-L-CD79b. Note intense
membrane staining of B cells. Paraffin section.
CD81
Clone 1D6
1 mL lyophilized NCL-CD81 P (HIER)
CD81 antigen, also known as TAPA-1, is a member of the TM4 superfamily
and is predicted to have four transmembrane regions, short cytoplasmic
N and C-termini and two extracellular regions. CD81 protein has a molecular
weight of 26 kD and is expressed by most cell types. Of the hematopoietic
cells, CD81 protein is reported to be expressed by B and T cells,
macrophages, dendritic cells, NK cells and eosinophils but not by
neutrophils, platelets or erythrocytes. The CD81 protein associates noncovalently
with a number of other molecules eg CD19, CD21, MHC class
I and II, CD20, CD37, CD53 and CD82 in B cells and CD4, CD8 and CD82 in
T cells. It also associates with the integrins CD29/CD49c (VLA-3), CD29/
CD49d (VLA-4) and CD29/CD49f (VLA-6) in several other cell types. No
extracellular ligand has been identified for CD81 protein and its function
remains unclear, although mouse CD81 protein plays a role in early T cell
development. The human CD81 molecule has been reported to be involved in
cell adhesion, motility, metastasis as well as cell activation and signal
transduction.
Human tonsil: immunohistochemical staining for CD81 antigen using NCL-CD81. Note
membrane staining of most cell types including B and T lymphocytes and vascular endothelial
cells. Paraffin section.
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This catalog is not for use in the USA.