Labelling Review row-Online

Primary Antibodies
Human cortex, Alzheimer's disease: immunohistochemical staining of amyloid precursor
protein using NCL-APP-228. Note intense staining of neurofibrillary tangles and senile plaques.
Paraffin section.
Anaplastic Lymphoma Kinase (ALK)
(CD246) (p80)
Clone 5A4
1 mL, 0.1 mL lyophilized NCL-ALK P (HIER)
7 mL Bond ready-to-use PA0306 P (HIER)
See also ALK (Anaplastic Lymphoma Kinase) (CD246) (p80) on page 54.
Androgen Receptor
Clone AR27
1 mL, 0.1 mL lyophilized NCL-AR-318 F P (HIER)
Clone 2F12
1 mL lyophilized NCL-AR-2F12 F P (HIER)
Clone AR27 was developed to produce superior staining to clone 2F12 on
paraffin sections.
Androgen Receptor is a member of the superfamily of ligand responsive
transcription regulators. The androgen receptor functions in the nucleus
where it is believed to act as a transcriptional regulator mediating the action
of male sex hormones (androgens). The androgen receptor has wide
distribution and can be demonstrated by immunohistochemistry in several
tissues eg prostate, skin, and oral mucosa. Androgen receptor has been
reported in a diverse range of human tumors eg osteosarcoma and in
prostatic carcinoma androgen receptor expression may be of clinical
relevance. Furthermore, mutation of the gene encoding androgen receptor
has been reported in prostatic carcinoma.
Human prostatic adenocarcinoma: immunohistochemical staining for androgen receptor using
NCL-AR-318. Note nuclear staining of tumor cells. Paraffin section.
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Reference Range
AP-2 Gamma
Clone GIA50
1 mL lyophilized NCL-AP2G P (HIER) W
The AP-2 transcription factors are required for normal growth and
morphogenesis during mammalian development. Initial in vitro studies have
also indicated that the AP-2 family of proteins are involved in the etiology of
human breast cancer. The various AP-2 genes are expressed in many
human breast cancer cell lines and critical AP-2 binding sites are present in
both c-erbB-2 and estrogen receptor promoters. AP-2 gamma has been
shown to be expressed in normal breast myoepithelial cells and to be
upregulated in a proportion of breast cancer specimens. AP-2 gamma
expression has been shown to be upregulated in the trophoblast lineage
throughout development, suggesting a crucial role for both trophoblast
development and differentiation. Gene expression and antibody studies have
indicated that AP-2 gamma expression occurs in testis within oogonia/
gonadocytes and was downregulated with germ cell differentiation. Several
studies have since indicated that AP-2 gamma may be useful in the
identification of testicular derived tumors.
Product Specific Information
NCL-AP2G has been shown, through immunohistochemistry, ELISA studies
and Western blotting to be specific for the AP-2 gamma transcription factor.
97.4 kD -
66.2 kD -
45.0 kD -
31.0 kD -
21.0 kD -
Western blot: detection of human AP2 gamma protein using NCL-AP2G. Lane A, molecular
weight markers. Lane B, SKBR3 cell line immunoblotted with NCL-AP2G.
APC (Adenomatous Polyposis Coli
Protein)
Clone EMM43
1 mL lyophilized NCL-APC P
The human adenomatous polyposis coli (APC) gene at locus 5q21 encodes a
protein of 2,843 amino acids. A precise role for APC in the regulation of the
wnt/beta-cateninin signalling pathway has been clearly recognized. APC
forms molecular complexes which are able to eliminate intra-cytoplasmic
beta-catening, inducing its degradation. It is expressed in the cytoplasm of
epithelial and mesenchymal cell types. In the epithelium of bladder, small
and large intestine, esophagus, stomach and epidermis, APC expression is
restricted to regions in which cell replication has ceased and terminal
differentiation has been established. Expression has been reported in lung,
kidney and mammary gland endothelial, myoepithelial and duct lining
epithelial cells. Some tissues such as ovary, myometrium, thyroid,
parathyroid and tonsil do not express the protein. Mutations of the APC gene
have been linked to the development of sporadic colorectal tumors, as well
as familial adenomatous polyposis and cancers of the pancreas, stomach
and esophagus. APC mutations have also been observed at significantly
high frequency in the advanced stages of breast cancer suggesting a
biological role in carcinogenesis.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
A B
- AP-2 gamma