Labelling Review row-Online

InhibinA
Clone AMY82 New!
1 mL, 0.1 mL liquid NCL-L-InhibinA P (HIER)
Inhibins and activins are members of the transforming growth factor beta
(TGF�) family of cytokines. Inhibins are heterodimers consisting of a
common �-subunit linked to either a �A subunit (�-�A, forming inhibin A) or
a �B subunit (�-�B, forming inhibin B). Activins share the �-subunit with the
inhibins and may be homo or heterodimers of �-subunits forming activin A
(�A-�A), activin AB (�A-�B) or activin B (�B-�B). The expression of the �subunit,
and therefore of inhibins appears to be more restricted than that of
the �-subunit, and therefore of activins. Inhibins and activins play a role in
the regulation of pituitary follicle stimulating hormone (FSH) secretion. The
actions of inhibins and activins are thought to oppose one another, with
inhibins suppressing FSH secretion and activins stimulating FSH secretion.
Inhibins are secreted by granulosa cells in female follicles and Sertoli cells
of the testis in the male. Inhibins are thought to have local regulatory roles in
a variety of tissues, in addition to the ovary, including the brain, adrenal
glands, bone marrow, fetus and placenta.
Human testis: immunohistochemical staining for Inhibin Alpha using NCL-L-InhibinA. Paraffin
section.
Insulin
Clone 2D11-H5
1 mL, 0.1 mL lyophilized NCL-INSULIN P
7 mL Bond ready-to-use PA0620 P
Insulin is a hormone secreted by the beta cells of the islets of Langerhans in
the pancreas. It promotes glycogen storage, formation of triglycerides, and
synthesis of protein and nucleic acids. Reports of immunocytochemical
investigation reveal the presence of insulin in the cytoplasm of certain islet
tumors. However, in some instances insulin-positive granules are sparse
and form a margin against the cell membrane.
Refer to page 32 for the Bond ready-to-use format.
Human pancreas: immunohistochemical staining for insulin-containing cells using NCL-
INSULIN. Note intense cytoplasmic staining of the beta cells of the islets of Langerhans and of
the tumor cells (center). Paraffin section.
Interleukin-2 Receptor (CD25)
Clone 4C9
1 mL, 0.1 mL lyophilized NCL-CD25-305 P (HIER)
7 mL Bond ready-to-use PA0368 P
CD25 antigen, the alpha subunit of interleukin-2 receptor, is a single-chain
glycoprotein with a molecular weight of 55 kD. Following the activation of T
cells with antigen or mitogen in the presence of the monokine interleukin-1,
interleukin-2 (IL-2) is rapidly synthesized and secreted. In response to this a
subpopulation of T cells expresses high affinity receptors for IL-2. These
cells proliferate, expanding the T cell population which is capable of
mediating helper, suppressor and cytotoxic functions. IL-2 receptor is not
exclusively found on T cells and is reported to be expressed on HTLVtransformed
T and B cells, EBV-transformed B cells, myeloid precursors and
oligodendrocytes. It is absent on thymocytes, resting T cells, non-activated
B cells and null cells. IL-2 receptor expression is reported to be associated
with inflammatory and malignant conditions, lymphoid neoplasia, autoimmune
diseases and allograft rejection.
Refer to page 21 for the Bond ready-to-use format.
Interleukin 6
Clone 10C12
1 mL, 0.1 mL liquid NCL-L-IL6 PW
IL-6 is a multifunctional cytokine that is secreted by both lymphoid and
nonlymphoid cells. It plays a key role in immune responses, hematopoiesis
and is an important cytokine in cell proliferation and differentiation. It may
also play an important role as an autocrine growth factor in metastatic
prostate cancer. IL-6 has been reported to play a role in secretion or release
of pituitary hormone in pituitary hormone secreting cells and adenomas. In
addition, IL-6 has been suggested to have a trophic effect in nerve cells and
to have a direct pathogenic role in CNS disorders. There are an increasing
number of reports that cytokines of the IL-6 family play an important
regulatory role in heart physiology.
Human colon: immunohistochemical staining for Interleukin 6 using NCL-L-IL6. Note
cytoplasmic staining of a proportion of lymphoid cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies